Comparative Metabolite Profiling Between and Other by Untargeted UHPLC-MS/MS

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2025 Feb 26

PMID 40001886

Authors

Jing Ma

Zhenjiang Chen

Kamran Malik

Chunjie Li

Affiliations

Soon will be listed here.

Abstract

is a second-class, nationally protected, medicinal fungi and serves as a functional nutrient in China. is extremely scarce due to its peculiar growing environment and the extensive gathering practices carried out by humans. A large number of counterfeit products for this fungi have also emerged in the market. At present, there is a lack of research on the differential metabolites of and its counterfeit products. The current study used an LC-MS non-targeted metabolomics method to compare the differences in metabolites between and other . The results indicated that there were significant differences in the metabolites between and the others. The 18 superclasses were found to have differences, involving lipids, organic acids, nucleosides, carbohydrates, amino acids, vitamins, and their derivatives. Compared with the other four groups of , 8 metabolites with significant differences were screened. In addition, the types and abundance of different metabolites of nucleosides of were superior compared to other (e.g., 5-Methyldioxycytidine, didanosine, cytidine, etc.). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the metabolism of arginine and proline, and glycerophosphate metabolism were the two significant differences in the metabolic pathways between and other . The research results provide a reference for identifying the authenticity of using non-targeted metabolic methods.

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