Patients With Immunoglobulin A Nephropathy Show Abnormal Frequencies of B Cell Subsets, Unconventional T Cells, and High Levels of Galactose-Deficient IgA1-Coated Gut Bacteria

Overview

Journal Kidney Int Rep

Publisher Elsevier

Specialty Nephrology

Date 2025 Feb 24

PMID 39990906

Authors

Micaela Gentile

Nina Goerlich

I-Ju Lo

N Eric Olson

Mark McConnell

Johannes Pospiech

Tobias Bohnenpoll

Philipp Skroblin

Olivier Radresa

Uwe Andag

Kirk N Campbell

Kristin Meliambro

Luis Sanchez-Russo

Alberto Verlato

Enrico Fiaccadori

Seunghee Kim-Schulze

Maria Lanau

M Loreto Fernandez-Lorente

Miguel Fribourg

Joaquin Manrique

Paolo Cravedi

Affiliations

Soon will be listed here.

Abstract

Introduction: Mucosal inflammation is involved in the pathophysiology of immunoglobulin-A nephropathy (IgAN); however, peripheral immune phenotype analyses of patients with IgAN often do not include unconventional T cells, the major subset in mucosal immunity.

Methods: We measured serum total IgA, galactose-deficient IgA1 (gd-IgA1), secretory IgA (SIgA), B cell-activating factor (BAFF), and A proliferation-inducing ligand (APRIL) in 66 patients with IgAN and 30 healthy controls (HCs). We also quantified the total IgA and gd-IgA1 in stool supernatant along with the same coated on bacteria. In 35 patients and 14 controls, we performed extensive phenotyping using cytometry by Time-of-Flight (CyTOF) of circulating immune cells, including unconventional T cells (mucosal associated invariant T [MAIT] cells, γδ T, and natural killer [NK] T cells). The results were validated using RNAseq data from a larger cohort of 179 patients with IgAN, 140 patients with minimal change disease, and 91 HCs.

Results: Patients with IgAN had higher circulating levels of total IgA, gd-IgA1, and APRIL, and higher IgA and gd-IgA1-coated gut bacteria than controls, whereas serum levels of SIgA and BAFF did not differ between groups. Patients with IgAN showed more class-switched memory (CSM) and double negative (DN) B cells than controls. MAIT cells and γδ T cells were significantly lower, and CD4CD8 NK T cells were significantly higher in patients with IgAN than in HCs. We validated the significant decrease in MAIT cells in an independent cohort of patients with IgAN.

Conclusion: The data indicate that patients with IgAN have increased circulating CSM and DN B cells associated with abnormal T cell immunity, involving defects in unconventional T cell frequency. This may suggest putative alterations at mucosal sites because of cell migration leading to altered IgA production.

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