Protocol for Circular Dichroism Spectral analysis of the thermal Stability Of CpG-methylated quadruplex Structures

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2025 Feb 22

PMID 39985771

Authors

Momo Moriya

Taiji Oyama

Masanori Goto

Kazunori Ikebukuro

Wataru Yoshida

Affiliations

Soon will be listed here.

Abstract

G-quadruplex and intercalated motif are quadruplex structures of which sequences are enriched in promoters. Here, we present a protocol for circular dichroism spectral analysis of the thermal stability of CpG-methylated quadruplex structures. We describe steps for preparing the oligonucleotide sample, measuring the circular dichroism spectrum of methylated quadruplex structures, and calculating thermodynamic parameters using Python 3. For complete details on the use and execution of this protocol, please refer to Kimura et al..

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