Fast Neural Population Dynamics in Primate V1 Captured by a Genetically-encoded Voltage Indicator

Overview

Journal Res Sq

Date 2025 Feb 20

PMID 39975906

Authors

Jingyang Zhou

Yuzhi Chen

Matt Whitmire

Pin Kwang Tan

Jimin Wu

Ashok Veeraraghavan

Jacob T Robinson

Jacob Robinson

Wilson Geisler

Vincent Pieribone

Vincent A Pieribone

Eyal Seidemann

Affiliations

Soon will be listed here.

Abstract

Genetically encoded voltage indicators (GEVIs) can measure millisecond-scale subthreshold neural responses with cell type specificity. Here, we successfully expressed, for the first time, a GEVI in excitatory V1 neurons in macaque monkeys. We then used widefield fluorescent imaging to measure V1 dynamics in response to visual stimuli with diverse temporal waveforms and contrasts, and compared these responses to signals measured using a genetically encoded calcium indicator (GECI) and a synthetic voltage-sensitive dye (VSD). When compared to GECI, GEVI captures faster response dynamics, tracks higher temporal frequencies, and responds to lower contrasts. To quantitatively characterize these three signals, we developed a simple nonlinear model that predicts the response dynamics to stimuli with arbitrary temporal waveforms and contrasts. Our results are consistent with the hypothesis that GEVI signals reflect the dynamics of locally summed membrane potentials, thus opening the door for a new class of experiments in behaving primates.

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