Transcriptional Control of T Cell Tissue Adaptation and Effector Function in Infants and Adults

Overview

Journal bioRxiv

Date 2025 Feb 20

PMID 39974963

Authors

Peter A Szabo

Hanna M Levitin

Thomas J Connors

David Chen

Jenny Jin

Puspa Thapa

Rebecca Guyer

Daniel P Caron

Joshua I Gray

Rei Matsumoto

Masaru Kubota

Maigan Brusko

Todd M Brusko

Donna L Farber

Peter A Sims

Affiliations

Soon will be listed here.

Abstract

The first years of life are essential for the development of memory T cells, which rapidly populate the body's diverse tissue sites during infancy. However, the degree to which tissue memory T cell responses in early life reflect those during adulthood is unclear. Here, we use single cell RNA-sequencing of resting and activated T cells from lymphoid and mucosal tissues of infant (aged 2-9 months) and adult (aged 40-65 years) human organ donors to dissect the transcriptional programming of memory T cells over age. Infant memory T cells demonstrate a unique stem-like transcriptional profile and tissue adaptation program, yet exhibit reduced activation capacity and effector function relative to adults. Using CRISPR-Cas9 knockdown, we define Helios () as a critical transcriptional regulator of the infant-specific tissue adaptation program and restricted effector state. Our findings reveal key transcriptional mechanisms that control tissue T cell fate and function in early life.

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