Development and Characterization of Calcium Ion-Enhanced Nanophytosomes Encapsulating Pomegranate Fruit Extract

Overview

Journal Food Sci Nutr

Specialty Biotechnology

Date 2025 Feb 17

PMID 39958255

Authors

Ramesh Sedighi

Ali Rafe

Ghadir Rajabzadeh

Abbas Pardakhty

Affiliations

Soon will be listed here.

Abstract

Nanophytosomes (NP) loaded with whole pomegranate fruit extract with peel and arils (PFE) at different levels of phosphatidylcholine (PC) were produced using a thin-film hydration method and reinforced with calcium ions. PFE was obtained by pressing whole pomegranates, followed by mixing with PC at ratios of 1:1, 1:2, and 1:3, which then strengthens the phytosome wall by CaCl solutions (1.35 and 2.70 mM) and lyophilized to create a stable powder form. The characteristics of the NP powders, including encapsulation efficiency (EE), particle size, ζ-potential, polydispersity index (PDI), structure, microstructure, and thermal properties, were evaluated. Additionally, the storage stability of phenolic compounds over two months was investigated. The PFE powder demonstrated appropriate characteristics for incorporation into the phytosome system, with a total phenol content of 371.19 mg GAE/g dry weight, anthocyanins at 300.68 mg/g, flavonoids at 194 mg/100 g, and an antioxidant activity of 90.98%. The highest EE was determined to be 98.53%, indicating its unique ability as a nano-carrier. PFE-loaded NPs showed favorable characteristics, such as low PDI values (< 0.5), smaller particle size (170 nm), and a spherical morphology. The PFE-NP had a particle size of 128.6 nm, zeta potential of -40.15 mV, mobility of -3.15 μm cm/Vs, PDI of 0.168, and EE of 98.53%. The optimized nanoparticles remained stable for two months at 4°C, with negligible changes in particle size (~10 nm), total phenol content (TPC), and PDI of the PFE-Nanophytosomes. All NP samples showed better stability at storage temperatures over 60 days. PEF-NPs improved the stability of phenolic compounds while improving solubility, masking taste, and delivery to target tissues, which can be considered in future applications.

References

Tan Q, Liu S, Chen X, Wu M, Wang H, Yin H . Design and evaluation of a novel evodiamine-phospholipid complex for improved oral bioavailability. AAPS PharmSciTech. 2012; 13(2):534-47. PMC: 3364372. DOI: 10.1208/s12249-012-9772-9. View

Andishmand H, Yousefi M, Jafari N, Azadmard-Damirchi S, Homayouni-Rad A, Torbati M . Designing and fabrication of colloidal nano-phytosomes with gamma-oryzanol and phosphatidylcholine for encapsulation and delivery of polyphenol-rich extract from pomegranate peel. Int J Biol Macromol. 2023; 256(Pt 2):128501. DOI: 10.1016/j.ijbiomac.2023.128501. View

Sze A, Erickson D, Ren L, Li D . Zeta-potential measurement using the Smoluchowski equation and the slope of the current-time relationship in electroosmotic flow. J Colloid Interface Sci. 2005; 261(2):402-10. DOI: 10.1016/S0021-9797(03)00142-5. View

Singh M, Lee K, Vinayagam R, Kang S . Antioxidant and Antibacterial Profiling of Pomegranate-pericarp Extract Functionalized-zinc Oxide Nanocomposite. Biotechnol Bioprocess Eng. 2021; 26(5):728-737. PMC: 8548265. DOI: 10.1007/s12257-021-0211-1. View

Kyriakoudi A, Spanidi E, Mourtzinos I, Gardikis K . Innovative Delivery Systems Loaded with Plant Bioactive Ingredients: Formulation Approaches and Applications. Plants (Basel). 2021; 10(6). PMC: 8234206. DOI: 10.3390/plants10061238. View

Nouri Z, Hajialyani M, Izadi Z, Bahramsoltani R, Farzaei M, Abdollahi M . Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review. Front Bioeng Biotechnol. 2020; 8:425. PMC: 7240035. DOI: 10.3389/fbioe.2020.00425. View

El Barnossi A, Moussaid F, Housseini A . Tangerine, banana and pomegranate peels valorisation for sustainable environment: A review. Biotechnol Rep (Amst). 2020; 29:e00574. PMC: 7758358. DOI: 10.1016/j.btre.2020.e00574. View

Human C, Aucamp M, de Beer D, Rijst M, Joubert E . Food-grade phytosome vesicles for nanoencapsulation of labile -glucosylated xanthones and dihydrochalcones present in a plant extract matrix-Effect of process conditions and stability assessment. Food Sci Nutr. 2023; 11(12):8093-8111. PMC: 10724603. DOI: 10.1002/fsn3.3730. View

Barbosa J, Abdelsadig M, Conway B, Merchant H . Using zeta potential to study the ionisation behaviour of polymers employed in modified-release dosage forms and estimating their pK. Int J Pharm X. 2019; 1:100024. PMC: 6733289. DOI: 10.1016/j.ijpx.2019.100024. View

10.

Zhu B, Zhou Q, Shi J, Shung K, Irisawa S, Takeuchi S . Self-separated hydrothermal lead zirconate titanate thick films for high frequency transducer applications. Appl Phys Lett. 2009; 94(10):102901. PMC: 2682757. DOI: 10.1063/1.3095504. View

11.

Lu M, Qiu Q, Luo X, Liu X, Sun J, Wang C . Phyto-phospholipid complexes (phytosomes): A novel strategy to improve the bioavailability of active constituents. Asian J Pharm Sci. 2020; 14(3):265-274. PMC: 7032241. DOI: 10.1016/j.ajps.2018.05.011. View

12.

Soltanzadeh M, Peighambardoust S, Ghanbarzadeh B, Mohammadi M, Lorenzo J . Chitosan nanoparticles encapsulating lemongrass (Cymbopogon commutatus) essential oil: Physicochemical, structural, antimicrobial and in-vitro release properties. Int J Biol Macromol. 2021; 192:1084-1097. DOI: 10.1016/j.ijbiomac.2021.10.070. View

13.

Li S, Wang C, Fu X, Li C, He X, Zhang B . Encapsulation of lutein into swelled cornstarch granules: Structure, stability and in vitro digestion. Food Chem. 2018; 268:362-368. DOI: 10.1016/j.foodchem.2018.06.078. View

14.

Andishmand H, Azadmard-Damirchi S, Hamishekar H, Torbati M, Kharazmi M, Savage G . Nano-delivery systems for encapsulation of phenolic compounds from pomegranate peel. Adv Colloid Interface Sci. 2023; 311:102833. DOI: 10.1016/j.cis.2022.102833. View

15.

Neslihan Dundar A, Ozdemir S, Uzuner K, Ekrem Parlak M, Irmak Sahin O, Dagdelen A . Characterization of pomegranate peel extract loaded nanophytosomes and the enhancement of bio-accessibility and storage stability. Food Chem. 2022; 398:133921. DOI: 10.1016/j.foodchem.2022.133921. View

16.

Wang Y, Xia S, Chen K, Zhang J, Tan H, Yu C . Atomic-Scale Tailoring C-N Coupling Sites for Efficient Acetamide Electrosynthesis over Cu-Anchored Boron Nitride Nanosheets. ACS Nano. 2024; 18(50):34403-34414. DOI: 10.1021/acsnano.4c14039. View

17.

Yang B, Dong Y, Wang F, Zhang Y . Nanoformulations to Enhance the Bioavailability and Physiological Functions of Polyphenols. Molecules. 2020; 25(20). PMC: 7587200. DOI: 10.3390/molecules25204613. View

18.

R Telange D, Patil A, Pethe A, Fegade H, Anand S, Dave V . Formulation and characterization of an apigenin-phospholipid phytosome (APLC) for improved solubility, in vivo bioavailability, and antioxidant potential. Eur J Pharm Sci. 2016; 108:36-49. DOI: 10.1016/j.ejps.2016.12.009. View