Antimicrobial Properties of Selected Microalgae Exopolysaccharide-enriched Extracts: Influence of Antimicrobial Assays and Targeted Microorganisms

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2025 Feb 12

PMID 39935647

Authors

Marion Pointcheval

Anthony Masse

David Flochlay

Franck Chanonat

Jacques Estival

Marie-Jose Durand

Affiliations

Soon will be listed here.

Abstract

Exopolysaccharide (EPS)-enriched extracts derived from microalgae exhibit a wide range of bioactive properties, including antibacterial and antifungal properties. However, these properties vary depending on the microalgae species, the antimicrobial assay used, and selected targeted microorganisms. This study offers to investigate the antimicrobial properties of exopolysaccharide-enriched extracts obtained from five microalgae species scarcely studied in this context. The targeted microorganisms selected for this study included Gram-positive () and Gram-negative bacteria (), fungi (), and microalgae (). Well-diffusion assay, broth microdilution assay, and growth measurements using absorbance were used to compare the methods and fully estimate the antimicrobial properties. Using absorbance measurements, growth rate inhibitions of at least 80% were observed on all targeted species for at least one microalgal EPS-enriched extract. At a concentration of 500 mgGlcEq · L, most active extracts of were obtained from (87.1% of growth inhibition), (53.7%), and (46.4%). EPS-enriched extracts from (86.2%), (59.9%), and (31.1%) were found to be the most effective against . Antifungal activities were the highest for EPS extracts from (86.0%), (16.6%), and (17.8%). The results indicated microalgae growth inhibition by EPS-enriched extracts from (99.3%), (84.8%), and (84.1%). To our knowledge, this study is the first to explore the algicidal properties of EPS-enriched extracts derived from microalgae, identifying promising candidates for future investigations into their potential applications.

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