Protocol for Profiling RNA MC Methylation at Base Resolution Using MC-TAC-seq

Overview

Journal STAR Protoc

Publisher Cell Press

Date 2025 Feb 2

PMID 39893640

Authors

Xiaoting Zhang

Liang Lu

Chengqi Yi

Xiaoyu Li

Affiliations

Soon will be listed here.

Abstract

RNA 5-methylcytosine (mC) is a widespread modification and plays a crucial role in gene expression regulation. Here, we present a protocol for transcriptome-wide mC methylome profiling at base resolution using bisulfite-free mC detection strategy enabled by ten-eleven translocation (TET)-assisted chemical labeling sequencing (mC-TAC-seq). We detail steps for TET-assisted chemical labeling, library construction, and data analysis. mC-TAC-seq enables accurate and robust mC detection in various RNA species. For complete details on the use and execution of this protocol, please refer to Lu et al..

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