Nucleus Pulposus Cell Network Modelling in the Intervertebral Disc

Overview

Journal NPJ Syst Biol Appl

Date 2025 Jan 31

PMID 39890859

Authors

Sofia Tseranidou

Maria Segarra-Queralt

Francis Kiptengwer Chemorion

Christine Lyn Le Maitre

Janet Pinero

Jerome Noailly

Affiliations

Soon will be listed here.

Abstract

Intervertebral disc degeneration (IDD) results from an imbalance between anabolic and catabolic processes in the extracellular matrix (ECM). Due to complex biochemical interactions, a comprehensive understanding is needed. This study presents a regulatory network model (RNM) for nucleus pulposus cells (NPC), representing normal intervertebral disc (IVD) conditions. The RNM includes 33 proteins, and 153 interactions based on literature, incorporating key NPC regulatory mechanisms. A semi-quantitative approach calculates the basal steady state, accurately reflecting normal NPC activity. Model validation through published studies replicated pro-catabolic and pro-anabolic shifts, emphasizing the roles of transforming growth factor beta (TGF-β) and interleukin-1 receptor antagonist (IL-1Ra) in ECM regulation. This IVD RNM is a valuable tool for predicting IDD progression, offering insights into ECM degradation mechanisms and guiding experimental research on IVD health and degeneration.

Citing Articles

Ex Vivo and In Vitro Proteomic Approach to Elucidate the Relevance of IL-4 and IL-10 in Intervertebral Disc Pathophysiology.

Bermudez-Lekerika P, Tseranidou S, Kanelis E, Nuesch A, Crump K, Alexopoulos L JOR Spine. 2025; 8(1):e70048.

PMID: 39931581 PMC: 11808320. DOI: 10.1002/jsp2.70048.

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