Evaluation of Single-dose Umbilical Cord Blood-derived Mononuclear Cell Injection Immediately and 7 Days After Spinal Cord Trauma in Mice

Overview

Journal Clinics (Sao Paulo)

Publisher Elsevier

Date 2025 Jan 31

PMID 39889361

Authors

Alex Oliveira de Araujo

Gustavo Bispo Dos Santos

Raphael Martus Marcon

Maria Helena Alves Nicola

Marcela Saldanha Pereira

Fernando Barbosa Sanchez

Thiego Pedro Freitas Araujo

Alexandre Fogaca Cristante

Affiliations

Soon will be listed here.

Abstract

Study Design: Experimental study utilizing a standardized Balb C mouse model.

Objectives: Evaluate histological changes and motor function recovery in the acute and subacute phases of Spinal Cord Injury (SCI) in mice using human Umbilical cord blood-derived mononuclear cells.

Methods: Forty mice were divided into five groups, with two receiving human Umbilical cord blood-derived mononuclear cells immediately after SCI and after 7 days, and three control groups. Motor assessment utilized BMS, MFS, and horizontal plane scales over six weeks. Necropsy evaluated macroscopic and histological spinal cord changes.

Results: Histologically, Umbilical cord blood-derived mononuclear cells-treated groups exhibited significant reductions in necrosis, hemorrhage, and degeneration compared to controls. Motor recovery showed partial improvement across all groups, with no statistically significant differences in scales between intervention and control groups.

Conclusions: In the acute phases of SCI, Umbilical cord blood-derived mononuclear cells applied directly to Balb C mice lesions demonstrated histological improvement but played a limited role in functional enhancement. The study highlights distinctions in the treatment's efficacy, potentially related to these cells' diverse differentiation capacities and intrinsic properties.

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