Allelopathic and Auto-toxic Effects of Aqueous Extracts of Codonopsis Pilosula Root Secretions

Overview

Journal PLoS One

Date 2025 Jan 31

PMID 39888897

Authors

Daiyu Qiu

Xue Wang

Fengbin Xu

Qian Li

Fengying Ren

Kan Jiang

Affiliations

Soon will be listed here.

Abstract

Water-soluble constituents in the root exudates of C. pilosula exhibit both allelopathic and auto-toxic properties, which substantially impede its growth. To mitigate the constraints associated with the continuous cropping of C. pilosula, this study examined the impact of aqueous extracts from the root exudates at various developmental stages on seed germination and seedling growth. Additionally, we isolated and characterized significant auto-toxic allelochemicals. Our findings revealed that the inhibitory effects on seed germination, seedling growth, and the activity of antioxidant enzymes-collectively referred to as a comprehensive effect-intensified progressively with increasing concentrations of the aqueous extracts from C. pilosula root exudates. The most pronounced auto-toxic comprehensive effect was observed with extracts at a concentration of 50 mL during the reproductive phase, diminishing SOD and POD activities, and MDA levels in C. pilosula seedlings by 72.3%, 71.7%, and 83.3% respectively, compared to the control (P<0.05). Three primary allelopathic compounds-acetic acid, hexamethyl cyclotrisiloxane, and methoxybenzene oxime were isolated and identified in the aqueous extracts. Acetic acid, constituting 44.1% of the total chemical profile, exhibited a significant inhibitory effect on seed germination and seedling growth (P<0.05). In conclusion, the aqueous extracts of C. pilosula root exudates significantly inhibit seed germination and seedling growth, especially during the plant's reproductive stage, with low molecular weight organic acids being the predominant allelopathic components.

References

Bai Y, Wang G, Cheng Y, Shi P, Yang C, Yang H . Soil acidification in continuously cropped tobacco alters bacterial community structure and diversity via the accumulation of phenolic acids. Sci Rep. 2019; 9(1):12499. PMC: 6715655. DOI: 10.1038/s41598-019-48611-5. View

Karimi E, Aliasgharzad N, Esfandiari E, Hassanpouraghdam M, Neu T, Buscot F . Biofilm forming rhizobacteria affect the physiological and biochemical responses of wheat to drought. AMB Express. 2022; 12(1):93. PMC: 9283637. DOI: 10.1186/s13568-022-01432-8. View

Zhang P, Xia L, Sun Y, Gao S . Soil nutrients and enzyme activities based on millet continuous cropping obstacles. Sci Rep. 2024; 14(1):17329. PMC: 11283559. DOI: 10.1038/s41598-024-68278-x. View

Wenzheng S, Guangrong S . General principle of high-quality academic development of traditional chinese medicine: "carrying on the essence, while pursuing innovations". J Tradit Chin Med. 2023; 43(1):1-2. PMC: 9924742. DOI: 10.19852/j.cnki.jtcm.2023.01.001. View

Yamamoto A, Seki M, Koseki K, Yabuta Y, Shimizu K, Arima J . Production and characterization of cyanocobalamin-enriched tomato (Solanum lycopersicum) fruits grown using hydroponics. J Sci Food Agric. 2022; 103(7):3685-3690. DOI: 10.1002/jsfa.12297. View

Makhwitine J, Kumalo H, Ndlovu S, Mkhwanazi N . Epigenetic Induction of Secondary Metabolites Production in Endophytic Fungi and GC-MS Analysis of Crude Metabolites with Anti-HIV-1 Activity. Microorganisms. 2023; 11(6). PMC: 10305084. DOI: 10.3390/microorganisms11061404. View

Dalluge J, Gort S, Hobson R, Selifonova O, Amore F, Gokarn R . Separation and identification of organic acid-coenzyme A thioesters using liquid chromatography/electrospray ionization-mass spectrometry. Anal Bioanal Chem. 2002; 374(5):835-40. DOI: 10.1007/s00216-002-1554-x. View

Liu L, Yang D, Liang T, Zhang H, He Z, Liang Z . Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots. Plant Cell Rep. 2016; 35(9):1933-42. DOI: 10.1007/s00299-016-2007-x. View

Ahmad A, Khan W, Shah A, Yasin N, Ali A, Rizwan M . Dopamine Alleviates Hydrocarbon Stress in Brassica oleracea through Modulation of Physio-Biochemical Attributes and Antioxidant Defense Systems. Chemosphere. 2020; 270:128633. DOI: 10.1016/j.chemosphere.2020.128633. View

Xie M, Yan Z, Ren X, Li X, Qin B . Codonopilate A, a Triterpenyl Ester as Main Autotoxin in Cultivated Soil of Codonopsis pilosula (Franch.) Nannf. J Agric Food Chem. 2017; 65(10):2032-2038. DOI: 10.1021/acs.jafc.6b04320. View

10.

Panchal P, Miller A, Giri J . Organic acids: versatile stress-response roles in plants. J Exp Bot. 2021; 72(11):4038-4052. DOI: 10.1093/jxb/erab019. View

11.

Tiwari H, Prajapati S . Allelopathic effect of benzoic acid (hydroponics root exudate) on microalgae growth. Environ Res. 2022; 219:115020. DOI: 10.1016/j.envres.2022.115020. View

12.

van Delden S, Nazarideljou M, Marcelis L . Nutrient solutions for : a study on nutrient solution composition in hydroponics systems. Plant Methods. 2020; 16:72. PMC: 7324969. DOI: 10.1186/s13007-020-00606-4. View

13.

Canarini A, Kaiser C, Merchant A, Richter A, Wanek W . Root Exudation of Primary Metabolites: Mechanisms and Their Roles in Plant Responses to Environmental Stimuli. Front Plant Sci. 2019; 10:157. PMC: 6407669. DOI: 10.3389/fpls.2019.00157. View

14.

Chen G, Xue Y, Yu X, Li C, Hou Y, Zhu H . The Structure and Function of Microbial Community in Rhizospheric Soil of American Ginseng (Panax quinquefolius L.) Changed with Planting Years. Curr Microbiol. 2022; 79(9):281. DOI: 10.1007/s00284-022-02941-2. View

15.

Armstrong J, Armstrong W . Rice and Phragmites: effects of organic acids on growth, root permeability, and radial oxygen loss to the rhizosphere. Am J Bot. 2011; 88(8):1359-70. View

16.

Ghimire B, Ghimire B, Yu C, Chung I . Allelopathic and Autotoxic Effects of -Derived Allelochemicals. Plants (Basel). 2019; 8(7). PMC: 6681227. DOI: 10.3390/plants8070233. View

17.

Yu J, Matsui Y . Phytotoxic substances in root exudates of cucumber (Cucumis sativus L.). J Chem Ecol. 2013; 20(1):21-31. DOI: 10.1007/BF02065988. View

18.

Xiang W, Chen J, Zhang F, Huang R, Li L . Autotoxicity in of root exudatesand their allelochemicals. Front Plant Sci. 2023; 13:1020626. PMC: 9807909. DOI: 10.3389/fpls.2022.1020626. View