Alleviate Serovar Typhimurium-induced Murine Inflammatory/oxidative Responses Via the P62-Keap1-Nrf2 Signaling Pathway and Cecal Microbiota

Overview

Journal Front Microbiol

Date 2025 Jan 31

PMID 39886212

Authors

Haihua Li

Xinyi Ma

Zhiyuan Shang

Xuejiao Liu

Jiayun Qiao

Affiliations

Soon will be listed here.

Abstract

Background: Serovar Typhimurium (. Typhimurium) infection can cause inflammation and oxidative stress in the body, leading to gastroenteritis, fever and other diseases in humans and animals. More and more studies have emphasized the broad prospects of probiotics in improving inflammation and oxidative stress, but the ability and mechanism of (LA) to alleviate the inflammatory/oxidative reaction caused by pathogens are still unclear.

Methods And Results: In this study, we treated the mice with LA for 14 days, infected them with . Typhimurium for 24 h, and sacrificed the mice to collect samples. We found that the early intervention of LA alleviated the pathological injury and reversed the down-regulation of the duodenal and hepatic tight junction protein mRNA levels caused by . Typhimurium infection. Compared with . Typhimurium group, LA early intervention increased the expression of antioxidant enzymes, but decreased the levels of serum malondialdehyde (MDA), interleukin-8 and tumor necrosis factor-α (TNF-α). Additionally, LA early intervention significantly increased mRNA expression in the liver and decreased mRNA expression in the duodenum compared to the . Typhimurium group. Furthermore, early LA treatment reduced the abundance of , increased the abundance of , and alleviated the decrease in SCFAs levels in the cecum of . Typhimurium-infected mice. Spearman correlation analysis showed that there was a certain correlation between cecal flora and serum indicators and short chain fatty acids.

Conclusion: Taken together, the results indicate that LA early intervention may alleviates . Typhimurium-induced inflammation and oxidative responses in mice by activating the p62-Keap1-Nrf2 signaling pathway and regulating the gut microbial community.

Significance And Impact Of The Study: Exploring the ability of LA to resist animal oxidative stress and microflora regulation caused by pathogenic microbes, so as to provide more options for developing healthy disease-resistant feed additives.

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