Enhancement of Titanium Surfaces Using Different Acid Solutions at Room Temperature to Improve Bone Cell Responses

Overview

Journal J Dent Sci

Date 2025 Jan 28

PMID 39873087

Authors

Thu Ya Linn

Eisner Salamanca

Chun-Yu Ho

Yi-Fan Wu

Hao-Chun Chiu

Wei-Jen Chang

Ying-Sui Sun

Affiliations

Soon will be listed here.

Abstract

Background/purpose: Early osseointegration of titanium (Ti) dental implants relies on the surface topography. Surface modification of Ti seeks to enhance bone regeneration around implants. Acid etching is the simple, less technique sensitive and cost-effective technique for surface treatment. The purpose of this study was to elucidate the simplified acid etching technique at room temperature lies in its capacity to enhance both physical properties and biological reactions relevant to bone.

Materials And Methods: Utilizing sulfuric acid (HSO) and hydrochloric acid (HCl), five distinct acid solutions were prepared, and the acid etching process was executed at five different time points at room temperature. The surface characterization of nanoscale modified titanium disks encompassed surface characteristics analysis, wettability and roughness tests. The biocompatibility evaluation involves tests that assess cell attachment, proliferation, alkaline phosphatase activity (ALP), and mineralization.

Results: The surface modified by HCl exhibited the most significant alterations, characterized by an elevated roughness value and reduced hydrophilicity properties. The surface treated with a mixture of HCl and HSO for 24 h (TH5) displayed a hydrophilic surface and high surface energy. Acid etched surfaces showed the greater cell attachment with long pseudopodia. The cell proliferation rate and ALP reaction rate of TH5 is the highest at day 7. Cells mineralization of Ti surface treated with 37% HCl for 24 h (TC5) shows the lowest and TH5 shows the greatest on day 21.

Conclusion: The proposed acid etching at room temperature utilizing a combination of HSO and HCl demonstrated improved physical properties while fostering favorable biological responses.

References

Bai Y, Wang L, Zhao L, Lingling E, Yang S, Jia S . Antibacterial and Antioxidant Effects of Magnesium Alloy on Titanium Dental Implants. Comput Math Methods Med. 2022; 2022:6537676. PMC: 8758302. DOI: 10.1155/2022/6537676. View

Cho S, Park K . The removal torque of titanium screw inserted in rabbit tibia treated by dual acid etching. Biomaterials. 2003; 24(20):3611-7. DOI: 10.1016/s0142-9612(03)00218-7. View

Iwaya Y, Machigashira M, Kanbara K, Miyamoto M, Noguchi K, Izumi Y . Surface properties and biocompatibility of acid-etched titanium. Dent Mater J. 2008; 27(3):415-21. DOI: 10.4012/dmj.27.415. View

Qi T, Weng J, Yu F, Zhang W, Li G, Qin H . Insights into the Role of Magnesium Ions in Affecting Osteogenic Differentiation of Mesenchymal Stem Cells. Biol Trace Elem Res. 2020; 199(2):559-567. DOI: 10.1007/s12011-020-02183-y. View

Buser D, Broggini N, Wieland M, Schenk R, Denzer A, Cochran D . Enhanced bone apposition to a chemically modified SLA titanium surface. J Dent Res. 2004; 83(7):529-33. DOI: 10.1177/154405910408300704. View

Shi G, Ren L, Wang L, Lin H, Wang S, Tong Y . H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and differentiation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108(3):368-75. DOI: 10.1016/j.tripleo.2009.05.033. View

Hung K, Lin Y, Feng H . The Effects of Acid Etching on the Nanomorphological Surface Characteristics and Activation Energy of Titanium Medical Materials. Materials (Basel). 2017; 10(10). PMC: 5666970. DOI: 10.3390/ma10101164. View

Rosa M, Albrektsson T, Francischone C, Filho H, Wennerberg A . The influence of surface treatment on the implant roughness pattern. J Appl Oral Sci. 2012; 20(5):550-5. PMC: 3881788. DOI: 10.1590/s1678-77572012000500010. View

Smith D, Zarb G . Criteria for success of osseointegrated endosseous implants. J Prosthet Dent. 1989; 62(5):567-72. DOI: 10.1016/0022-3913(89)90081-4. View

10.

Guler B, Uraz A, Hatipoglu H, Yalim M . Chemical Evaluation of Energy Dispersive X-ray Spectroscopy Analysis of Different Failing Dental Implant Surfaces: A Comparative Clinical Trial. Materials (Basel). 2021; 14(4). PMC: 7923284. DOI: 10.3390/ma14040986. View

11.

Chen C, Ikeuchi Y, Xu L, Sewvandi G, Kusunose T, Tanaka Y . Synthesis of [111]- and {010}-faceted anatase TiO2 nanocrystals from tri-titanate nanosheets and their photocatalytic and DSSC performances. Nanoscale. 2015; 7(17):7980-91. DOI: 10.1039/c5nr00069f. View

12.

Jemat A, Ghazali M, Razali M, Otsuka Y . Surface Modifications and Their Effects on Titanium Dental Implants. Biomed Res Int. 2015; 2015:791725. PMC: 4575991. DOI: 10.1155/2015/791725. View

13.

Klokkevold P, Nishimura R, Adachi M, Caputo A . Osseointegration enhanced by chemical etching of the titanium surface. A torque removal study in the rabbit. Clin Oral Implants Res. 1998; 8(6):442-7. DOI: 10.1034/j.1600-0501.1997.080601.x. View

14.

Nicolas-Silvente A, Velasco-Ortega E, Ortiz-Garcia I, Monsalve-Guil L, Gil J, Jimenez-Guerra A . Influence of the Titanium Implant Surface Treatment on the Surface Roughness and Chemical Composition. Materials (Basel). 2020; 13(2). PMC: 7014346. DOI: 10.3390/ma13020314. View

15.

Lin X, Zhou L, Li S, Lu H, Ding X . Behavior of acid etching on titanium: topography, hydrophility and hydrogen concentration. Biomed Mater. 2013; 9(1):015002. DOI: 10.1088/1748-6041/9/1/015002. View

16.

Albrektsson T, Wennerberg A . On osseointegration in relation to implant surfaces. Clin Implant Dent Relat Res. 2019; 21 Suppl 1:4-7. DOI: 10.1111/cid.12742. View

17.

Kieswetter K, Schwartz Z, Hummert T, Cochran D, Simpson J, Dean D . Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater Res. 1996; 32(1):55-63. DOI: 10.1002/(SICI)1097-4636(199609)32:1<55::AID-JBM7>3.0.CO;2-O. View