[Effects of LncRNA SNHG20 on Epithelial Mesenchymal Transition and Microtubule Formation in Human Oral Squamous Cell Carcinoma Cells Through Targeted Regulation of the MiR-520c-3p/ Pathway]

Overview

Journal Beijing Da Xue Xue Bao Yi Xue Ban

Date 2025 Jan 24

PMID 39856503

Authors

Minying Ma

Xiaoqin Chao

Yang Zhao

Guoting Zhao

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the effects of LncRNA SNHG20 on epithelial mesenchymal transition (EMT) and microtubule formation in human oral squamous cell carcinoma (OSCC) cells through targeted regulation of the miR-520c-3p/ pathway.

Methods: After real-time fluorescence quantitative detection of LncRNA SNHG20, miR-520c-3p, mRNA expression levels in OSCC tissues and cells, dual luciferase reporter assay was used to detect the relationship between the three. OSCC cells were randomly separated into control group, sh-NC group, sh-SNHG20 group, sh-SNHG20+anti NC group, and sh-SNHG20+anti miR-520c-3p group. Western blotting was used to detect the expression of N-cadherin, vimentin, and E-cadherin proteins in the OSCC cells. The morphology of HSC-3 cells was observed under microscope. Changes in the number of microtubules formed were detected. The effect of LncRNA SNHG20 on the growth of OSCC tumors and the expression levels of LncRNA SNHG20, miR-520c-3p and RAB22 A in the transplanted tumors were detected by nude mice tumorigenesis experiment.

Results: LncRNA SNHG20 and mRNA were upregulated in the OSCC tissues and cells, while miR-520c-3p was downregulated ( < 0.05). There were binding sites between LncRNA SNHG20 and miR-520c-3p, RAB22A and miR-520c-3p, which had targeted regulation relationship. Compared with the sh-NC group, the sh-SNHG20 group had fewer stromal like cells, more epithelial like cells, incomplete microtubule structure, and fewer nodules. LncRNA SNHG20, RAB22A, N-Cadherin, and vimentin were downregulated, while miR-520c-3p and E-cadherin were upregulated ( < 0.05). Compared with the sh-SNHG20+anti-NC group, the sh-SNHG20+anti-miR-520c-3p group had a higher number of stromal like cells, a lower number of epithelioid cells, tighter microtubule arrangement, and more microtubule nodules. miR-520c-3p and E-cadherin were downregulated, while RAB22A, N-cadherin, and vimentin were upregulated ( < 0.05). The transplanted tumor of OSCC in sh-SNHG20 group was smaller and lower than that in sh-NC group. The expression levels of LncRNA SNHG20 and RAB22A in the transplanted tumor tissues were lower than those in sh-NC group, and the expression level of miR-520c-3p was higher than that in sh-NC group ( < 0.05).

Conclusion: LncRNA SNHG20 promotes epithelial-mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells by targeting the miR-520c-3p/ pathway. Inhibiting the expression of LncRNA SNHG20 can target and regulate the miR-520c-3p/ pathway to inhibit EMT and microtubule formation in OSCC cells.

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