LncRNA FER1L4 Promotes Oral Squamous Cell Carcinoma Progression Via Targeting MiR-133a-5p/Prx1 Axis

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2021 Feb 11

PMID 33568918

Citations 10

Authors

Nan Zhang

Lingfang Zeng

Shouyi Wang

Ronghua Wang

Rui Yang

Zuolin Jin

Hong Tao

Affiliations

Soon will be listed here.

Abstract

Background: Oral squamous cell carcinoma (OSCC) is a common cancer especially young people in the world. The long non-coding RNA Fer-1-like protein 4 (FER1L4) has been reported to be closely associated with the progression of various human cancers. However, the role of FER1L4 in OSCC remains unclear.

Methods: The expression level of FER1L4 in OSCC tissues and cancer cell lines was detected by using quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was evaluated by cell counting kit-8 (CCK-8) assay and EdU staining assay. Cell invasion and migration were evaluated by Transwell assay. Cell apoptosis was detected by flow cytometry. Luciferase reporter assay was performed to determine the targeting relationship between FER1L4, miR-133a-5p and Prx1. The protein expression of Prx1 was detected by Western blot. In addition, a xenograft tumor model in vivo was constructed to confirm the function of FER1L4.

Results: FERIL4 was significantly upregulated in OSCC tissues and cancer cell lines. Moreover, high level of FER1L4 predicted a poor prognosis of OSCC patients. Silencing of FER1L4 not only significantly inhibited cell growth, invasion, migration and induced apoptosis in SCC-9 and HN4 cells in vitro, but also effectively suppressed the tumorigenesis of OSCC cells in vivo. Knockdown of FER1L4 significantly enhanced the expression of miR-133a-5p by sponging it, and then downregulated Prx1 expression.

Conclusion: Our study elucidated a new mechanism of lncRNA FER1L4 that promoting OSCC progression by directly targeting miR-133a-5p/Prx1 axis and provided novel therapeutic targets for OSCC.

Citing Articles

[Effects of LncRNA SNHG20 on epithelial mesenchymal transition and microtubule formation in human oral squamous cell carcinoma cells through targeted regulation of the miR-520c-3p/ pathway].

Ma M, Chao X, Zhao Y, Zhao G Beijing Da Xue Xue Bao Yi Xue Ban. 2025; 57(1):26-32.

PMID: 39856503 PMC: 11759804.

Epigenetic mechanism of RBM15 in affecting cisplatin resistance in laryngeal carcinoma cells by regulating ferroptosis.

Liang Y, Zhong H, Zhao Y, Tang X, Pan C, Sun J Biol Direct. 2024; 19(1):57.

PMID: 39039611 PMC: 11264397. DOI: 10.1186/s13062-024-00499-6.

ENAH-202 promotes cancer progression in oral squamous cell carcinoma by regulating ZNF502/VIM axis.

Zhang X, Chen X, Sun D, Song N, Li M, Zheng W Cancer Med. 2023; 12(22):20892-20905.

PMID: 37902191 PMC: 10709750. DOI: 10.1002/cam4.6652.

A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma.

Xu W, Lou W, Mei L Epigenetics. 2022; 18(1):2148433.

PMID: 36476264 PMC: 9980666. DOI: 10.1080/15592294.2022.2148433.

Circulating Long Non-Coding RNAs Could Be the Potential Prognostic Biomarker for Liquid Biopsy for the Clinical Management of Oral Squamous Cell Carcinoma.

Dey Ghosh R, Guha Majumder S Cancers (Basel). 2022; 14(22).

PMID: 36428681 PMC: 9688117. DOI: 10.3390/cancers14225590.

References

Chen F, Qi S, Zhang X, Wu J, Yang X, Wang R . lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/β‑catenin pathway in oral squamous cell carcinoma. Int J Oncol. 2019; 54(4):1183-1194. PMC: 6411352. DOI: 10.3892/ijo.2019.4707. View

Yang Y, Chen D, Liu H, Yang K . Increased expression of lncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma. Cell Death Dis. 2019; 10(2):41. PMC: 6381212. DOI: 10.1038/s41419-018-1280-8. View

Hong Y, He H, Sui W, Zhang J, Zhang S, Yang D . Long non-coding RNA H1 promotes cell proliferation and invasion by acting as a ceRNA of miR‑138 and releasing EZH2 in oral squamous cell carcinoma. Int J Oncol. 2018; 52(3):901-912. DOI: 10.3892/ijo.2018.4247. View

Cox A, Tolkach Y, Kristiansen G, Ritter M, Ellinger J . The lncRNA Fer1L4 is an adverse prognostic parameter in clear-cell renal-cell carcinoma. Clin Transl Oncol. 2020; 22(9):1524-1531. PMC: 7381450. DOI: 10.1007/s12094-020-02291-0. View

Wang C, Niu W, Chen H, Shi N, He D, Zhang M . Nicotine suppresses apoptosis by regulating α7nAChR/Prx1 axis in oral precancerous lesions. Oncotarget. 2017; 8(43):75065-75075. PMC: 5650400. DOI: 10.18632/oncotarget.20506. View

Fang Z, Zhao J, Xie W, Sun Q, Wang H, Qiao B . LncRNA UCA1 promotes proliferation and cisplatin resistance of oral squamous cell carcinoma by sunppressing miR-184 expression. Cancer Med. 2017; 6(12):2897-2908. PMC: 5727307. DOI: 10.1002/cam4.1253. View

Kessler P, Grabenbauer G, Leher A, Bloch-Birkholz A, Vairaktaris E, Neukam F . Neoadjuvant and adjuvant therapy in patients with oral squamous cell carcinoma Long-term survival in a prospective, non-randomized study. Br J Oral Maxillofac Surg. 2007; 46(1):1-5. DOI: 10.1016/j.bjoms.2007.08.006. View

Kong Y, Feng Y, Xiao Y, Liu S, Li X, Yang Q . LncRNA LUCAT1 promotes rowth, migration, and invasion of oral squamous cell carcinoma by upregulating PCNA. Eur Rev Med Pharmacol Sci. 2019; 23(11):4770-4776. DOI: 10.26355/eurrev_201906_18059. View

Zhang W, Wu Y, Shiozaki Y, Sugimoto Y, Takigawa T, Tanaka M . miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3' UTR of RUNX2. DNA Cell Biol. 2018; 37(3):199-209. DOI: 10.1089/dna.2017.3936. View

10.

Ma W, Zhang C, Li H, Gu J, Miao G, Cai H . LncRNA FER1L4 suppressed cancer cell growth and invasion in esophageal squamous cell carcinoma. Eur Rev Med Pharmacol Sci. 2018; 22(9):2638-2645. DOI: 10.26355/eurrev_201805_14958. View

11.

Liu Z, Shao Y, Tan L, Shi H, Chen S, Guo J . Clinical significance of the low expression of FER1L4 in gastric cancer patients. Tumour Biol. 2014; 35(10):9613-7. DOI: 10.1007/s13277-014-2259-4. View

12.

Niu W, Zhang M, Chen H, Wang C, Shi N, Jing X . Peroxiredoxin 1 promotes invasion and migration by regulating epithelial-to-mesenchymal transition during oral carcinogenesis. Oncotarget. 2016; 7(30):47042-47051. PMC: 5216922. DOI: 10.18632/oncotarget.9705. View

13.

Zhang C, Bao C, Zhang X, Lin X, Pan D, Chen Y . Knockdown of lncRNA LEF1-AS1 inhibited the progression of oral squamous cell carcinoma (OSCC) via Hippo signaling pathway. Cancer Biol Ther. 2019; 20(9):1213-1222. PMC: 6741770. DOI: 10.1080/15384047.2019.1599671. View

14.

Feng X, Luo Q, Zhang H, Wang H, Chen W, Meng G . The role of NLRP3 inflammasome in 5-fluorouracil resistance of oral squamous cell carcinoma. J Exp Clin Cancer Res. 2017; 36(1):81. PMC: 5479028. DOI: 10.1186/s13046-017-0553-x. View

15.

Gao X, Wang N, Wu S, Cui H, An X, Yang Y . Long non‑coding RNA FER1L4 inhibits cell proliferation and metastasis through regulation of the PI3K/AKT signaling pathway in lung cancer cells. Mol Med Rep. 2019; 20(1):182-190. PMC: 6579969. DOI: 10.3892/mmr.2019.10219. View

16.

Wang X, Dong K, Jin Q, Ma Y, Yin S, Wang S . Upregulation of lncRNA FER1L4 suppresses the proliferation and migration of the hepatocellular carcinoma via regulating PI3K/AKT signal pathway. J Cell Biochem. 2018; 120(4):6781-6788. DOI: 10.1002/jcb.27980. View

17.

Huang W, Cao J, Peng X . LINC01234 facilitates growth and invasiveness of oral squamous cell carcinoma through regulating the miR-637/NUPR1 axis. Biomed Pharmacother. 2019; 120:109507. DOI: 10.1016/j.biopha.2019.109507. View

18.

Wang Y, Zhang X, Wang Z, Hu Q, Wu J, Li Y . LncRNA-p23154 promotes the invasion-metastasis potential of oral squamous cell carcinoma by regulating Glut1-mediated glycolysis. Cancer Lett. 2018; 434:172-183. DOI: 10.1016/j.canlet.2018.07.016. View

19.

Vermorken J, Mesia R, Rivera F, Remenar E, Kawecki A, Rottey S . Platinum-based chemotherapy plus cetuximab in head and neck cancer. N Engl J Med. 2008; 359(11):1116-27. DOI: 10.1056/NEJMoa0802656. View

20.

Zhang X, Feng H, Li Z, Guo J, Li M . Aspirin is Involved in the Cell Cycle Arrest, Apoptosis, Cell Migration, and Invasion of Oral Squamous Cell Carcinoma. Int J Mol Sci. 2018; 19(7). PMC: 6073368. DOI: 10.3390/ijms19072029. View