In Vivo Calcium Extrusion from Accumbal Astrocytes Reduces Anxiety-like Behaviors but Increases Compulsive-like Responses and Compulsive Ethanol Drinking in Mice

Overview

Journal Neuropharmacology

Date 2025 Jan 22

PMID 39842625

Authors

Lee Peyton

Humza Haroon

Anthony Umpierre

Hesham Essa

Robert Bruce

Long-Jun Wu

Doo-Sup Choi

Affiliations

Soon will be listed here.

Abstract

The ventral striatum is crucially involved in reward processing. The present study investigates the behavioral effects of astrocyte-specific calcium extrusion virus "CalEx" on perseverative responses in the operant five-choice serial reaction time task and ethanol-conditioned place preference. Mice were injected with CalEx via the GfaABCD promoter to extrude cytosolic calcium from astrocytes within the ventral striatum. We found that CalEx transfection in the ventral striatum reduced evoked response duration, the maximum amplitude, and the response frequency to 500 μM ATP as measured by ΔF/F fluorescence intensity of the genetically encoded calcium indicator targeting astrocytes GCaMP6f. During the five-choice serial reaction time task, CalEx mice persisted in perseverative responses compared to their counterparts. Additionally, during ethanol-conditioned place preference, CalEx mice showed increased place preference for a low ethanol concentration compared to control group. Furthermore, we found that accumbal astrocytic calcium extrusion increased quinine adulterated ethanol drinking. Our findings suggest that diminishing ventral striatum astrocyte calcium activity contributes to compulsive behaviors, ethanol drinking, and enhanced ethanol drug reward.

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