Comprehensive Genome Annotation of Trilocha Varians, a New Model Species of Lepidopteran Insects

Overview

Journal Sci Data

Date 2025 Jan 21

PMID 39837902

Authors

Jung Lee

Toshiaki Fujimoto

Katsushi Yamaguchi

Shuji Shigenobu

Ken Sahara

Toru Shimada

Affiliations

Soon will be listed here.

Abstract

Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotation information on the chromosome-scale assembly of T. varians previously published by our group. 9 RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction. As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6% of BUSCO sequences were present in our gene models. ATAC-seq was also conducted to determine chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed T. varians genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential users who plan to use this species.

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