Extraction Efficiency and Bioactive Evaluation of Tamarix Nilotica and Arthrocnemum Macrostachyum Extracts for Anti-cancer Potential

Overview

Journal PLoS One

Date 2025 Jan 21

PMID 39836644

Authors

Maryam Al Kaabi

Nancy A ElNaker

Nila Jan

Michael A Ochsenkuhn

Shady A Amin

Lina F Yousef

Ahmed F Yousef

Affiliations

Soon will be listed here.

Abstract

This study aimed to evaluate the potential of phytochemicals from two native UAE plant species, Arthrocnemum macrostachyum and Tamarix nilotica, as anti-cancer agents. The plant extracts were obtained using two methods, maceration, and microwave-assisted extraction (MAE), and were subsequently evaluated for their in vitro cytotoxicity against three cancer cell lines: breast (MDA-MB-231), colon (HCT-116), and lung (A-549). Results suggest that: 1) MAE is more efficient than maceration in recovering metabolites from plant biomass based on measurements of total phenolic content, radical scavenging activity, and bioactivity of extracts based on in vitro cytotoxicity. 2) Only T. nilotica extracts were found to be bioactive based on cytotoxicity measurements. 3) Cancer cell lines displayed differential sensitivity to T. nilotica crude extracts, with breast cancer cells being the most sensitive and lung cancer cells being the least sensitive. 4) Solid-phase fractionation of T. nilotica crude extract using different percentages of methanol resulted in several fractions that were 100-fold more cytotoxic compared to the crude unfractionated extract. The 30% and 70% methanol fractions exhibited the highest cytotoxicity towards breast and colon cancer cell lines, respectively. 5) Untargeted metabolomics using UHPLC-Q-ToF-MS of T. nilotica crude extracts revealed 909 molecular features, of which only 327 were annotated using MS/MS fragmentation. The results suggest that T. nilotica extracts have potential as anti-cancer agents and that MAE is an efficient method for extracting phytochemicals from plant biomass. The study also revealed that cancer cell lines exhibited differential sensitivity to the extracts and that solid-phase fractionation of crude extract using different percentages of methanol can yield fractions that are more cytotoxic than the crude extract.

References

Basu S, Duren W, Evans C, Burant C, Michailidis G, Karnovsky A . Sparse network modeling and metscape-based visualization methods for the analysis of large-scale metabolomics data. Bioinformatics. 2017; 33(10):1545-1553. PMC: 5860222. DOI: 10.1093/bioinformatics/btx012. View

Kumar S, Priyatharshni S, Babu V, Mangalaraj D, Viswanathan C, Kannan S . Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications. J Colloid Interface Sci. 2014; 436:234-42. DOI: 10.1016/j.jcis.2014.08.064. View

Takagi M, Shin-Ya K . Construction of a natural product library containing secondary metabolites produced by actinomycetes. J Antibiot (Tokyo). 2012; 65(9):443-7. DOI: 10.1038/ja.2012.52. View

Cragg G, Newman D . Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005; 100(1-2):72-9. DOI: 10.1016/j.jep.2005.05.011. View

Hrelia S, Bordoni A, Biagi P, Rossi C, Bernardi L, Horrobin D . gamma-Linolenic acid supplementation can affect cancer cell proliferation via modification of fatty acid composition. Biochem Biophys Res Commun. 1996; 225(2):441-7. DOI: 10.1006/bbrc.1996.1192. View

Yoshihara S, Kon A, Kudo D, Nakazawa H, Kakizaki I, Sasaki M . A hyaluronan synthase suppressor, 4-methylumbelliferone, inhibits liver metastasis of melanoma cells. FEBS Lett. 2005; 579(12):2722-6. DOI: 10.1016/j.febslet.2005.03.079. View

Sanchez-Tena S, Fernandez-Cachon M, Carreras A, Mateos-Martin M, Costoya N, Moyer M . Hamamelitannin from witch hazel (Hamamelis virginiana) displays specific cytotoxic activity against colon cancer cells. J Nat Prod. 2012; 75(1):26-33. DOI: 10.1021/np200426k. View

Ranjan Patra C, Mukherjee S, Kotcherlakota R . Biosynthesized silver nanoparticles: a step forward for cancer theranostics?. Nanomedicine (Lond). 2014; 9(10):1445-8. DOI: 10.2217/nnm.14.89. View

Zhang A, Sun H, Wang P, Han Y, Wang X . Modern analytical techniques in metabolomics analysis. Analyst. 2011; 137(2):293-300. DOI: 10.1039/c1an15605e. View

El-Gewely M . Dysregulation of Regulatory ncRNAs and Diseases. Int J Mol Sci. 2024; 25(1). PMC: 10778827. DOI: 10.3390/ijms25010024. View

10.

Probst C, Parry C, Wittchen H, Rehm J . The socioeconomic profile of alcohol-attributable mortality in South Africa: a modelling study. BMC Med. 2018; 16(1):97. PMC: 6016129. DOI: 10.1186/s12916-018-1080-0. View

10.

Lokeshwar V, Lopez L, Munoz D, Chi A, Shirodkar S, Lokeshwar S . Antitumor activity of hyaluronic acid synthesis inhibitor 4-methylumbelliferone in prostate cancer cells. Cancer Res. 2010; 70(7):2613-23. PMC: 2848908. DOI: 10.1158/0008-5472.CAN-09-3185. View

11.

Belhaj M, Lawler N, Hoffman N . Metabolomics and Lipidomics: Expanding the Molecular Landscape of Exercise Biology. Metabolites. 2021; 11(3). PMC: 8001908. DOI: 10.3390/metabo11030151. View

12.

Kudo D, Kon A, Yoshihara S, Kakizaki I, Sasaki M, Endo M . Effect of a hyaluronan synthase suppressor, 4-methylumbelliferone, on B16F-10 melanoma cell adhesion and locomotion. Biochem Biophys Res Commun. 2004; 321(4):783-7. DOI: 10.1016/j.bbrc.2004.07.041. View

13.

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M . Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2014; 136(5):E359-86. DOI: 10.1002/ijc.29210. View

14.

Janecka I . Cancer control through principles of systems science, complexity, and chaos theory: a model. Int J Med Sci. 2007; 4(3):164-73. PMC: 1891444. DOI: 10.7150/ijms.4.164. View

15.

Ahmad A, Sarkar S, Aboukameel A, Ali S, Biersack B, Seibt S . Anticancer action of garcinol in vitro and in vivo is in part mediated through inhibition of STAT-3 signaling. Carcinogenesis. 2012; 33(12):2450-6. DOI: 10.1093/carcin/bgs290. View

16.

Orabi M, Zidan S, Attia G, Alyami H, Matsunami K, Hatano T . Ellagitannins and simple phenolics from the halophytic plant . Nat Prod Res. 2020; 36(1):177-185. DOI: 10.1080/14786419.2020.1774757. View

17.

Newman D, Cragg G . Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod. 2012; 75(3):311-35. PMC: 3721181. DOI: 10.1021/np200906s. View

17.

Fazekas D, Koltai M, Turei D, Modos D, Palfy M, Dul Z . SignaLink 2 - a signaling pathway resource with multi-layered regulatory networks. BMC Syst Biol. 2013; 7:7. PMC: 3599410. DOI: 10.1186/1752-0509-7-7. View