Identification of Chilli Genome Encoded MicroRNAs Targeting the 16S RRNA and Genes of " Phytoplasma Trifolii

Overview

Journal Front Bioinform

Date 2025 Jan 21

PMID 39834655

Authors

Vineeta Pandey

Aarshi Srivastava

Ramwant Gupta

Haitham E M Zaki

Muhammad Shafiq Shahid

Rajarshi K Gaur

Affiliations

Soon will be listed here.

Abstract

Phytoplasma, a potentially hazardous pathogen associated with witches' broom, is an economically harmful disease-producing bacteria that damages chilli cultivation. Phytoplasma-infected plants display various symptoms that indicate significant disruptions in normal plant physiology and behaviour. Diseases caused by phytoplasma are widespread and have a major economic impact on crop quality and yield. This work focuses on identifying and examining chilli microRNAs (miRNAs) as potential targets against the 16S rRNA and gene of " Phytoplasma trifolii" (". P. trifolii") through plant miRNA prediction algorithms. Mature chilli miRNAs (CA-miRNAs) were collected and used to hybridise the 16S rRNA and genes. A total of four common CA-miRNAs were picked according to genetic consensus. Three algorithms applied in the present study suggested that the physiologically relevant, top-ranked miR169b_2 has a possibly specific site at nucleotide position 1,006 for targeting the '. P. trifolii' 16S rRNA gene. The circos algorithm was then utilised to create the miRNA-mRNA regulatory network. The free energy between the miRNA:mRNA duplex was also computed, and the best value of -17.46 kcal/mol was obtained for CA-miR166c_2. Currently, there are no suitable commercial '. P. trifolii'-resistant chilli crops. As a result, the expected biological data provide useful evidence for developing '. P. trifolii'-resistant chilli plants.

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