RNAi-Based Antiviral Innate Immunity in Plants

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Feb 26

PMID 35216025

Authors

Liying Jin

Mengna Chen

Meiqin Xiang

Zhongxin Guo

Affiliations

Soon will be listed here.

Abstract

Multiple antiviral immunities were developed to defend against viral infection in hosts. RNA interference (RNAi)-based antiviral innate immunity is evolutionarily conserved in eukaryotes and plays a vital role against all types of viruses. During the arms race between the host and virus, many viruses evolve viral suppressors of RNA silencing (VSRs) to inhibit antiviral innate immunity. Here, we reviewed the mechanism at different stages in RNAi-based antiviral innate immunity in plants and the counteractions of various VSRs, mainly upon infection of RNA viruses in model plant Arabidopsis. Some critical challenges in the field were also proposed, and we think that further elucidating conserved antiviral innate immunity may convey a broad spectrum of antiviral strategies to prevent viral diseases in the future.

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References

Jaubert M, Bhattacharjee S, Mello A, Perry K, Moffett P . ARGONAUTE2 mediates RNA-silencing antiviral defenses against Potato virus X in Arabidopsis. Plant Physiol. 2011; 156(3):1556-64. PMC: 3135937. DOI: 10.1104/pp.111.178012. View

Hafren A, Ustun S, Hochmuth A, Svenning S, Johansen T, Hofius D . Turnip Mosaic Virus Counteracts Selective Autophagy of the Viral Silencing Suppressor HCpro. Plant Physiol. 2017; 176(1):649-662. PMC: 5761789. DOI: 10.1104/pp.17.01198. View

Jiang N, Gutierrez-Diaz A, Mukundi E, Lee Y, Meyers B, Otegui M . Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of Arabidopsis carbon metabolism. Nat Commun. 2020; 11(1):2456. PMC: 7229025. DOI: 10.1038/s41467-020-16289-3. View

Baumberger N, Baulcombe D . Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Proc Natl Acad Sci U S A. 2005; 102(33):11928-33. PMC: 1182554. DOI: 10.1073/pnas.0505461102. View

Mosher R, Schwach F, Studholme D, Baulcombe D . PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis. Proc Natl Acad Sci U S A. 2008; 105(8):3145-50. PMC: 2268599. DOI: 10.1073/pnas.0709632105. View

Zhu H, Hu F, Wang R, Zhou X, Sze S, Liou L . Arabidopsis Argonaute10 specifically sequesters miR166/165 to regulate shoot apical meristem development. Cell. 2011; 145(2):242-56. PMC: 4124879. DOI: 10.1016/j.cell.2011.03.024. View

Li F, Pignatta D, Bendix C, Brunkard J, Cohn M, Tung J . MicroRNA regulation of plant innate immune receptors. Proc Natl Acad Sci U S A. 2012; 109(5):1790-5. PMC: 3277104. DOI: 10.1073/pnas.1118282109. View

Ren B, Guo Y, Gao F, Zhou P, Wu F, Meng Z . Multiple functions of Rice dwarf phytoreovirus Pns10 in suppressing systemic RNA silencing. J Virol. 2010; 84(24):12914-23. PMC: 3004324. DOI: 10.1128/JVI.00864-10. View

Xie M, Zhang S, Yu B . microRNA biogenesis, degradation and activity in plants. Cell Mol Life Sci. 2014; 72(1):87-99. PMC: 11113746. DOI: 10.1007/s00018-014-1728-7. View

10.

Borges F, Martienssen R . The expanding world of small RNAs in plants. Nat Rev Mol Cell Biol. 2015; 16(12):727-41. PMC: 4948178. DOI: 10.1038/nrm4085. View

11.

Li F, Wang Y, Zhou X . SGS3 Cooperates with RDR6 in Triggering Geminivirus-Induced Gene Silencing and in Suppressing Geminivirus Infection in Nicotiana Benthamiana. Viruses. 2017; 9(9). PMC: 5618013. DOI: 10.3390/v9090247. View

12.

Mello C, Conte Jr D . Revealing the world of RNA interference. Nature. 2004; 431(7006):338-42. DOI: 10.1038/nature02872. View

13.

Wang W, Ye R, Xin Y, Fang X, Li C, Shi H . An importin β protein negatively regulates MicroRNA activity in Arabidopsis. Plant Cell. 2011; 23(10):3565-76. PMC: 3229135. DOI: 10.1105/tpc.111.091058. View

14.

Bortolamiol D, Pazhouhandeh M, Marrocco K, Genschik P, Ziegler-Graff V . The Polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing. Curr Biol. 2007; 17(18):1615-21. DOI: 10.1016/j.cub.2007.07.061. View

15.

Carthew R, Sontheimer E . Origins and Mechanisms of miRNAs and siRNAs. Cell. 2009; 136(4):642-55. PMC: 2675692. DOI: 10.1016/j.cell.2009.01.035. View

16.

Merai Z, Kerenyi Z, Molnar A, Barta E, Valoczi A, Bisztray G . Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity. J Virol. 2005; 79(11):7217-26. PMC: 1112109. DOI: 10.1128/JVI.79.11.7217-7226.2005. View

17.

Goswami S, Sahana N, Pandey V, Doblas P, Jain R, Palukaitis P . Interference in plant defense and development by non-structural protein NSs of Groundnut bud necrosis virus. Virus Res. 2011; 163(1):368-73. DOI: 10.1016/j.virusres.2011.08.016. View

18.

Ma X, Nicole M, Meteignier L, Hong N, Wang G, Moffett P . Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. J Exp Bot. 2014; 66(3):919-32. DOI: 10.1093/jxb/eru447. View

19.

Huang C, Wang H, Hu P, Hamby R, Jin H . Small RNAs - Big Players in Plant-Microbe Interactions. Cell Host Microbe. 2019; 26(2):173-182. DOI: 10.1016/j.chom.2019.07.021. View

20.

Valli A, Martin-Hernandez A, Lopez-Moya J, Garcia J . RNA silencing suppression by a second copy of the P1 serine protease of Cucumber vein yellowing ipomovirus, a member of the family Potyviridae that lacks the cysteine protease HCPro. J Virol. 2006; 80(20):10055-63. PMC: 1617295. DOI: 10.1128/JVI.00985-06. View