Analysis of the Effect of ALA-PDT on Macrophages in Footpad Model of Mice Infected with Based on Single-cell Sequencing

Overview

Journal Open Med (Wars)

Date 2025 Jan 17

PMID 39822988

Authors

Wenyi Chen

Xuelin Wu

Muhammad Danish Yaqoob

Kangxing Liu

Yanqing Hu

Xiuling Ke

Yongxuan Hu

Affiliations

Soon will be listed here.

Abstract

Chromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in the recent era for the treatment of CBM and has exhibited promising effects in several clinical case reports. We established a mice footpad infection model with and analyzed the impact of PDT treatment on the immune response of macrophages using single-cell sequencing. The results showed that infection of the mouse footpad skin with results in an increase in inflammatory cells, primarily mononuclear-macrophages, with the activation of complement and enhancement of cell chemotaxis, leading to upregulation of anti-infection-related pathways. After ALA-PDT treatment, the number of inflammatory cells decreased, while macrophages upregulated the expression of antigen-recognition-related genes, enhancing phagocytosis and autophagy-related biological functions.

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