A Human Oral Commensal-mediated Protection Against Sjögren's Syndrome with Maintenance of T Cell Immune Homeostasis and Improved Oral Microbiota

Overview

Journal NPJ Biofilms Microbiomes

Date 2025 Jan 17

PMID 39820778

Authors

Yu-Chao Tseng

Kai-Sheng Liao

Wei-Ting Lin

Chin Li

Chia-Bin Chang

Jie-Wei Hsu

Chin-Pui Chan

Chun-Ming Chen

Hon-Pin Wang

Hsiu-Chuan Chien

Jann-Tay Wang

Song-Chou Hsieh

Shu-Fen Wu

Affiliations

Soon will be listed here.

Abstract

Sjögren's syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women's health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenzae in SS. Reduced salivary H. parainfluenzae levels in SS patients were confirmed through quantitative PCR. Oral H. parainfluenzae inoculation in NOD mice alleviated focal sialadenitis, improved salivary function, and reduced IFN-γCD3 and IFN-γCD8 T cells in salivary gland-draining lymph nodes, maintaining immune homeostasis against a biased type 1 response. Inoculation also enhanced salivary microbiota diversity, balanced the Firmicutes-to-Proteobacteria ratio, and reduced the overwhelming presence of Pseudomonas mendocina. In vitro, H. parainfluenzae-preconditioned A253 cells limited CD8 T cell expansion with reduced IFN-γ production. These findings suggest that H. parainfluenzae improves oral microbial diversity, promotes homeostatic T-cell immunity, and protects against SS, supporting its potential as a next-generation probiotic.

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