Quality Assurance in Metabolomics and Metabolic Profiling

Overview

Journal Methods Mol Biol

Date 2025 Jan 15

PMID 39812975

Authors

Jennifer A Kirwan

Ulrike Bruning

Jonathan D Mosley

Affiliations

Soon will be listed here.

Abstract

Metabolic profiling (untargeted metabolomics) aims for a global unbiased analysis of metabolites in a cell or biological system. It remains a highly useful research tool used across various analytical platforms. Incremental improvements across multiple steps in the analytical process may have large consequences for the end quality of the data. Thus, this chapter concentrates on which aspects of quality assurance can be implemented by a lab in the (pre-)analytical stages of the analysis to improve the overall end quality of their data. The scope of this chapter is limited to liquid-chromatography-mass spectrometry (LC-MS)-based profiling, which is one of the most widely utilized platforms, although the general principles are applicable to all metabolomics experiments.

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