The Potential of the Probiotic Isolate SS18-50 to Prevent Colitis in Mice

Overview

Journal Food Sci Nutr

Date 2025 Jan 13

PMID 39803293

Authors

Dan Wang

Tiehua Zhang

Luxin Qiu

Changhui Zhao

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to investigate the effect of the () SS18-50 (an isolate with favorable probiotic properties following space traveling) on dextran sulfate sodium (DSS)-induced colitis in mice. Male ICR mice were randomly assigned to one of six groups: a control group, a model group, and four intervention groups comprising the isolate (SS18-50-L and SS18-50-H) and the wild type (GS18-L and GS18-H) strains. The model group and the intervention groups were administered a 3.5% DSS (w/v) solution to induce acute enteritis. The four intervention groups were administered the corresponding bacterial suspensions, SS18-50-L (1.0 × 10 CFU/mL), SS18-50-H (1.0 × 10 CFU/mL), GS18-L (1.0 × 10 CFU/mL), and GS18-H (1.0 × 10 CFU/mL). The results demonstrated that the disease activity index (DAI) score of the SS18-50-H was markedly lower than that of the CON. Subsequently, the colon tissue of mice was analyzed to determine the levels of myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA). The results demonstrated that all strains within the intervention groups exhibited good performance to prevent colitis. Particularly, the SS18-50-H strain exhibited a pronounced stimulative effect on GSH, an increase in SOD activity, and a decrease in MPO activity and MDA content. The SS18-50-H treatment resulted in a notable elevation in serum somatostatin (SS) levels and a concomitant reduction in endothelin (ET) and substance P (SP) levels, which approached normal ranges. The results of the RT-qPCR analysis demonstrated that the mRNA expression levels of tumor necrosis factor (TNF-α), cyclooxygenase (COX-2), interleukin (IL-10), and interleukin (IL-6) in the SS18-50-H were significantly reduced to levels comparable to those observed in the CON. In conclusion, SS18-50 has been demonstrated to inhibit the development of colitis in a dose-dependent manner, thereby establishing it as a high-quality lactic acid bacterium with a colitis inhibitory effect.

References

Rodriguez-Nogales A, Algieri F, Garrido-Mesa J, Vezza T, Utrilla M, Chueca N . Differential intestinal anti-inflammatory effects of Lactobacillus fermentum and Lactobacillus salivarius in DSS mouse colitis: impact on microRNAs expression and microbiota composition. Mol Nutr Food Res. 2017; 61(11). DOI: 10.1002/mnfr.201700144. View

Zheng B, van Bergenhenegouwen J, van de Kant H, Folkerts G, Garssen J, Vos A . Specific probiotic dietary supplementation leads to different effects during remission and relapse in murine chronic colitis. Benef Microbes. 2015; 7(2):205-13. DOI: 10.3920/bm2015.0037. View

Wan J, Zhang J, Yin H, Chen D, Yu B, He J . Ameliorative effects of alginate oligosaccharide on tumour necrosis factor-α-induced intestinal epithelial cell injury. Int Immunopharmacol. 2020; 89(Pt B):107084. DOI: 10.1016/j.intimp.2020.107084. View

Sun M, Zhang F, Yin X, Cheng B, Zhao C, Wang Y . Lactobacillus reuteri F-9-35 Prevents DSS-Induced Colitis by Inhibiting Proinflammatory Gene Expression and Restoring the Gut Microbiota in Mice. J Food Sci. 2018; 83(10):2645-2652. DOI: 10.1111/1750-3841.14326. View

Youn H, Kim H, Kim H, Seo K . A comparative evaluation of the kefir yeast A4 and sulfasalazine in ulcerative colitis: anti-inflammatory impact and gut microbiota modulation. Food Funct. 2024; 15(12):6717-6730. DOI: 10.1039/d4fo00427b. View

Camuesco D, Comalada M, Rodriguez-Cabezas M, Nieto A, Lorente M, Concha A . The intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression. Br J Pharmacol. 2004; 143(7):908-18. PMC: 1575937. DOI: 10.1038/sj.bjp.0705941. View

Sommer J, Engelowski E, Baran P, Garbers C, Floss D, Scheller J . Interleukin-6, but not the interleukin-6 receptor plays a role in recovery from dextran sodium sulfate-induced colitis. Int J Mol Med. 2014; 34(3):651-60. PMC: 4121342. DOI: 10.3892/ijmm.2014.1825. View

Kim S, Guevarra R, Kim Y, Kwon J, Kim H, Cho J . Role of Probiotics in Human Gut Microbiome-Associated Diseases. J Microbiol Biotechnol. 2019; 29(9):1335-1340. DOI: 10.4014/jmb.1906.06064. View

Lee H, Ahn Y, Lee J, Huh C, Kim D . Evaluation of anti-colitic effect of lactic acid bacteria in mice by cDNA microarray analysis. Inflammation. 2009; 32(6):379-86. DOI: 10.1007/s10753-009-9146-y. View

10.

Derwa Y, Gracie D, Hamlin P, Ford A . Systematic review with meta-analysis: the efficacy of probiotics in inflammatory bowel disease. Aliment Pharmacol Ther. 2017; 46(4):389-400. DOI: 10.1111/apt.14203. View

11.

Zhu H, Robert Li Y . Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: updated experimental and clinical evidence. Exp Biol Med (Maywood). 2012; 237(5):474-80. DOI: 10.1258/ebm.2011.011358. View

12.

Kai N, Qingsong C, Kejia M, Weiwei L, Xing W, Xuejie C . An Inflammatory Bowel Diseases Integrated Resources Portal (IBDIRP). Database (Oxford). 2024; 2024. PMC: 10791110. DOI: 10.1093/database/baad097. View

13.

Wittmann Dayagi T, Werner L, Pinsker M, Salamon N, Barschak I, Weiss B . Mucosal IL-10 and IL-10 receptor expression patterns in paediatric patients with ulcerative colitis. Int J Exp Pathol. 2021; 102(1):4-10. PMC: 7839950. DOI: 10.1111/iep.12382. View

14.

Cordeiro B, Alves J, Belo G, Oliveira E, Braga M, da Silva S . Therapeutic Effects of Probiotic Minas Frescal Cheese on the Attenuation of Ulcerative Colitis in a Murine Model. Front Microbiol. 2021; 12:623920. PMC: 7960676. DOI: 10.3389/fmicb.2021.623920. View

15.

Niu X, Li Q, Luan N, Liu J, Zhang M, An J . BW2013 protects mucosal integrity and modulates gut microbiota of mice with colitis. Can J Microbiol. 2023; 69(4):158-169. DOI: 10.1139/cjm-2022-0092. View

16.

Cooper H, Murthy S, Shah R, Sedergran D . Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69(2):238-49. View

17.

Angerio A, Bufalino D, Bresnick M, Bell C, Brill S . Inflammatory bowel disease and endothelin-1: a review. Crit Care Nurs Q. 2005; 28(2):208-13. DOI: 10.1097/00002727-200504000-00013. View

18.

Le Berre C, Honap S, Peyrin-Biroulet L . Ulcerative colitis. Lancet. 2023; 402(10401):571-584. DOI: 10.1016/S0140-6736(23)00966-2. View

19.

Burns P, Alard J, Hrd J, Boutillier D, Paez R, Reinheimer J . Spray-drying process preserves the protective capacity of a breast milk-derived Bifidobacterium lactis strain on acute and chronic colitis in mice. Sci Rep. 2017; 7:43211. PMC: 5324110. DOI: 10.1038/srep43211. View

20.

Viennois E, Chen F, Laroui H, Baker M, Merlin D . Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNA. BMC Res Notes. 2013; 6:360. PMC: 3847706. DOI: 10.1186/1756-0500-6-360. View