London Rocket ( L.) As Healthy Green: Bioactive Compounds and Bioactivity of Plants Grown in Wild and Controlled Environments

Overview

Journal Molecules

Publisher MDPI

Date 2025 Jan 11

PMID 39795089

Authors

Tarik Chileh-Chelh

Tatiana Pagan Loeiro da Cunha-Chiamolera

Miguel Urrestarazu

Mohamed Ezzaitouni

Rosalia Lopez-Ruiz

Cinthia Najera

Miguel Angel Rincon-Cervera

Jose Luis Guil-Guerrero

Affiliations

Soon will be listed here.

Abstract

London rocket () is a wild green consumed globally, yet its phytochemical composition remains underexplored. In this study, we analyzed the leaves of wild plants and those grown in controlled environments (GCE) with varying electrical conductivities (EC) and light spectra. Plants were assessed for growth, phenolic content, vitamin C, antioxidant activity, glucosinolates, and antiproliferative effects against HT-29 human colorectal cancer cells. The optimal biomass yield occurred at the EC levels of 3.0-3.5 dS m under Valoya LED light. Wild plants showed higher antioxidant activity (DPPH and ABTS assays) than GCE samples, with values of 8.03-8.67 and 6.49-6.81 mmol TE per 100 g dry weight, respectively. The vitamin C range was 50.7-84.3 and 84.5-186.9 mg 100 g fresh weight for GCE and wild samples, respectively. Phenolic content was higher in wild plants than in the GCE ones, with apigetrin as the primary phenolic compound. The MTT assay showed that ethanol extracts from wild plants weakly inhibited HT-29 cell growth, with a GI of 210-380 µg mL after 72 h of cells exposure to plant extracts. Principal Component Analysis suggested that EC and UV exposure increase the antioxidant activity, total phenolics, and glucosinolates in wild plants, offering insights into the bioactive profiles of leaves.

References

Marcum C, Jarrell T, Zhu H, Owen B, Haupert L, Easton M . A Fundamental Tandem Mass Spectrometry Study of the Collision-Activated Dissociation of Small Deprotonated Molecules Related to Lignin. ChemSusChem. 2016; 9(24):3513-3526. DOI: 10.1002/cssc.201600678. View

Sadeer N, Montesano D, Albrizio S, Zengin G, Mahomoodally M . The Versatility of Antioxidant Assays in Food Science and Safety-Chemistry, Applications, Strengths, and Limitations. Antioxidants (Basel). 2020; 9(8). PMC: 7464350. DOI: 10.3390/antiox9080709. View

Arnon D, Johnson C . INFLUENCE OF HYDROGEN ION CONCENTRATION ON THE GROWTH OF HIGHER PLANTS UNDER CONTROLLED CONDITIONS. Plant Physiol. 1942; 17(4):525-39. PMC: 438054. DOI: 10.1104/pp.17.4.525. View

Najera C, Ros M, Moreno D, Hernandez-Lara A, Pascual J . Combined effect of an agro-industrial compost and light spectra composition on yield and phytochemical profile in mizuna and pak choi microgreens. Heliyon. 2024; 10(4):e26390. PMC: 10901005. DOI: 10.1016/j.heliyon.2024.e26390. View

Rincon-Cervera M, da Cunha-Chiamolera T, Chileh-Chelh T, Carmona-Fernandez M, Urrestarazu M, Guil-Guerrero J . Growth parameters, phytochemicals, and antitumor activity of wild and cultivated ice plants ( L.). Food Sci Nutr. 2024; 12(9):6548-6562. PMC: 11561852. DOI: 10.1002/fsn3.4286. View

Bhosale P, Abusaliya A, Kim H, Ha S, Park M, Jeong S . Apigetrin Promotes TNFα-Induced Apoptosis, Necroptosis, G2/M Phase Cell Cycle Arrest, and ROS Generation through Inhibition of NF-κB Pathway in Hep3B Liver Cancer Cells. Cells. 2022; 11(17). PMC: 9454891. DOI: 10.3390/cells11172734. View

Han H, Kim J, Lee H . Effect of apigetrin in pseudo-SARS-CoV-2-induced inflammatory and pulmonary fibrosis in vitro model. Sci Rep. 2024; 14(1):14545. PMC: 11196261. DOI: 10.1038/s41598-024-65447-w. View

Sun Q, Lu N, Feng L . Apigetrin inhibits gastric cancer progression through inducing apoptosis and regulating ROS-modulated STAT3/JAK2 pathway. Biochem Biophys Res Commun. 2018; 498(1):164-170. DOI: 10.1016/j.bbrc.2018.02.009. View

Calvo M, Martin-Diana A, Rico D, Lopez-Caballero M, Martinez-Alvarez O . Antioxidant, Antihypertensive, Hypoglycaemic and Nootropic Activity of a Polyphenolic Extract from the Halophyte Ice Plant (). Foods. 2022; 11(11). PMC: 9180490. DOI: 10.3390/foods11111581. View

10.

Liang Y, Zhao W, Wang C, Wang Z, Wang Z, Zhang J . A Comprehensive Screening and Identification of Genistin Metabolites in Rats Based on Multiple Metabolite Templates Combined with UHPLC-HRMS Analysis. Molecules. 2018; 23(8). PMC: 6222673. DOI: 10.3390/molecules23081862. View

11.

El-Seedi H, Burman R, Mansour A, Turki Z, Boulos L, Gullbo J . The traditional medical uses and cytotoxic activities of sixty-one Egyptian plants: discovery of an active cardiac glycoside from Urginea maritima. J Ethnopharmacol. 2012; 145(3):746-57. DOI: 10.1016/j.jep.2012.12.007. View

12.

Fabre N, Rustan I, de Hoffmann E, Quetin-Leclercq J . Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry. J Am Soc Mass Spectrom. 2001; 12(6):707-15. DOI: 10.1016/S1044-0305(01)00226-4. View

13.

Signore A, Serio F, Santamaria P . A Targeted Management of the Nutrient Solution in a Soilless Tomato Crop According to Plant Needs. Front Plant Sci. 2016; 7:391. PMC: 4876364. DOI: 10.3389/fpls.2016.00391. View

14.

Guil J, Rodriguez-Garcia I, Torija E . Nutritional and toxic factors in selected wild edible plants. Plant Foods Hum Nutr. 1997; 51(2):99-107. DOI: 10.1023/a:1007988815888. View

15.

Velasco P, Francisco M, Moreno D, Ferreres F, Garcia-Viguera C, Cartea M . Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics. Phytochem Anal. 2011; 22(2):144-52. DOI: 10.1002/pca.1259. View

16.

Maina S, Ryu D, Cho J, Jung D, Park J, Nho C . Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of L. Microgreens. Antioxidants (Basel). 2021; 10(8). PMC: 8389028. DOI: 10.3390/antiox10081183. View

17.

Abranko L, Szilvassy B . Mass spectrometric profiling of flavonoid glycoconjugates possessing isomeric aglycones. J Mass Spectrom. 2015; 50(1):71-80. DOI: 10.1002/jms.3474. View

18.

Zorzan M, Zucca P, Collazuol D, Peddio S, Rescigno A, Pezzani R . Sisymbrium officinale, the Plant of Singers: A Review of Its Properties and Uses. Planta Med. 2020; 86(5):307-311. DOI: 10.1055/a-1088-9928. View

19.

Abellan A, Dominguez-Perles R, Garcia-Viguera C, Moreno D . In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts. Nutrients. 2021; 13(11). PMC: 8619005. DOI: 10.3390/nu13114140. View

20.

Borgonovo G, Zimbaldi N, Guarise M, Bedussi F, Winnig M, Vennegeerts T . Glucosinolates in (L.) Scop.: Comparative Analysis in Cultivated and Wild Plants and in Vitro Assays with T2Rs Bitter Taste Receptors. Molecules. 2019; 24(24). PMC: 6943552. DOI: 10.3390/molecules24244572. View