Quadruplet Codon Decoding-based Versatile Genetic Biocontainment System

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Date 2025 Jan 8

PMID 39777466

Authors

Yun-Nam Choi

Donghyeon Kim

Seongbeom Lee

Ye Rim Shin

Jeong Wook Lee

Affiliations

Soon will be listed here.

Abstract

Biological resources, such as sequence information, genetic traits, materials and strains, pose risks when inadvertently released or deliberately misused. To address these concerns, we developed Quadruplet COdon DEcoding (QCODE), a versatile genetic biocontainment strategy that introduces a quadruplet codon (Q-codon) causing frameshifts, hindering proper gene expression. Strategically incorporating Q-codons in multiple genes prevents genetic trait escape, unallowed proliferation of microbial strains and unauthorized leakages of genetic materials. This multifaceted strategy, integrating Q-codons for genetic traits, materials and strains, ensures robust biocontainment across various levels. Notably, our system maintains sequence protection, safeguarding genetic sequence information against unauthorized access. The QCODE approach offers a versatile, efficient and compact solution to enhance biosecurity in diverse biological research settings.

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