Advancements in Nanohydroxyapatite: Synthesis, Biomedical Applications and Composite Developments

Overview

Journal Regen Biomater

Date 2025 Jan 8

PMID 39776858

Authors

Rui Zhao

Xiang Meng

Zixian Pan

Yongjia Li

Hui Qian

Xiangdong Zhu

Xiao Yang

Xingdong Zhang

Affiliations

Soon will be listed here.

Abstract

Nanohydroxyapatite (nHA) is distinguished by its exceptional biocompatibility, bioactivity and biodegradability, qualities attributed to its similarity to the mineral component of human bone. This review discusses the synthesis techniques of nHA, highlighting how these methods shape its physicochemical attributes and, in turn, its utility in biomedical applications. The versatility of nHA is further enhanced by doping with biologically significant ions like magnesium or zinc, which can improve its bioactivity and confer therapeutic properties. Notably, nHA-based composites, incorporating metal, polymeric and bioceramic scaffolds, exhibit enhanced osteoconductivity and osteoinductivity. In orthopedic field, nHA and its composites serve effectively as bone graft substitutes, showing exceptional osteointegration and vascularization capabilities. In dentistry, these materials contribute to enamel remineralization, mitigate tooth sensitivity and are employed in surface modification of dental implants. For cancer therapy, nHA composites offer a promising strategy to inhibit tumor growth while sparing healthy tissues. Furthermore, nHA-based composites are emerging as sophisticated platforms with high surface ratio for the delivery of drugs and bioactive substances, gradually releasing therapeutic agents for progressive treatment benefits. Overall, this review delineates the synthesis, modifications and applications of nHA in various biomedical fields, shed light on the future advancements in biomaterials research.

References

He Y, Lv C, Hou X, Wu L . Mono-dispersed nano-hydroxyapatite based MRI probe with tetrahedral DNA nanostructures modification for in vitro tumor cell imaging. Anal Chim Acta. 2020; 1138:141-149. DOI: 10.1016/j.aca.2020.09.006. View

Lee M, Seonwoo H, Jang K, Pandey S, Lim J, Park S . Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells. Bioact Mater. 2021; 6(9):2742-2751. PMC: 7895645. DOI: 10.1016/j.bioactmat.2021.01.020. View

Nie L, Zhang H, Ren A, Li Y, Fu G, Cannon R . Nano-hydroxyapatite mineralized silk fibroin porous scaffold for tooth extraction site preservation. Dent Mater. 2019; 35(10):1397-1407. DOI: 10.1016/j.dental.2019.07.024. View

Ding L, Han S, Wang K, Zheng S, Zheng W, Peng X . Remineralization of enamel caries by an amelogenin-derived peptide and fluoride . Regen Biomater. 2020; 7(3):283-292. PMC: 7266664. DOI: 10.1093/rb/rbaa003. View

Sundarabharathi L, Parangusan H, Ponnamma D, Al-Maadeed M, Chinnaswamy M . In-vitro biocompatibility, bioactivity and photoluminescence properties of Eu /Sr dual-doped nano-hydroxyapatite for biomedical applications. J Biomed Mater Res B Appl Biomater. 2017; 106(6):2191-2201. DOI: 10.1002/jbm.b.34023. View

Coelho C, Grenho L, Gomes P, Quadros P, Fernandes M . Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment. Sci Rep. 2019; 9(1):11050. PMC: 6667430. DOI: 10.1038/s41598-019-47491-z. View

Amaechi B, Lemke K, Saha S, Luong M, Gelfond J . Clinical efficacy of nanohydroxyapatite-containing toothpaste at relieving dentin hypersensitivity: an 8 weeks randomized control trial. BDJ Open. 2021; 7(1):23. PMC: 8233401. DOI: 10.1038/s41405-021-00080-7. View

Barros J, Ferraz M, Azeredo J, Fernandes M, Gomes P, Monteiro F . Alginate-nanohydroxyapatite hydrogel system: Optimizing the formulation for enhanced bone regeneration. Mater Sci Eng C Mater Biol Appl. 2019; 105:109985. DOI: 10.1016/j.msec.2019.109985. View

Wang J, Tang Y, Cao Q, Wu Y, Wang Y, Yuan B . Fabrication and biological evaluation of 3D-printed calcium phosphate ceramic scaffolds with distinct macroporous geometries through digital light processing technology. Regen Biomater. 2022; 9:rbac005. PMC: 9160879. DOI: 10.1093/rb/rbac005. View

10.

Resende R, Sartoretto S, Uzeda M, Alves A, Calasans-Maia J, Rossi A . Randomized Controlled Clinical Trial of Nanostructured Carbonated Hydroxyapatite for Alveolar Bone Repair. Materials (Basel). 2019; 12(22). PMC: 6887796. DOI: 10.3390/ma12223645. View

11.

Han Y, Li S, Cao X, Yuan L, Wang Y, Yin Y . Different inhibitory effect and mechanism of hydroxyapatite nanoparticles on normal cells and cancer cells in vitro and in vivo. Sci Rep. 2014; 4:7134. PMC: 4238015. DOI: 10.1038/srep07134. View

12.

Liu Q, Zhao Y, Zhou H, Chen C . Ferroptosis: challenges and opportunities for nanomaterials in cancer therapy. Regen Biomater. 2023; 10:rbad004. PMC: 9926950. DOI: 10.1093/rb/rbad004. View

13.

Imran E, Cooper P, Ratnayake J, Ekambaram M, Mei M . Potential Beneficial Effects of Hydroxyapatite Nanoparticles on Caries Lesions In Vitro-A Review of the Literature. Dent J (Basel). 2023; 11(2). PMC: 9955150. DOI: 10.3390/dj11020040. View

14.

Hassani A, Khoshfetrat A, Rahbarghazi R, Sakai S . Collagen and nano-hydroxyapatite interactions in alginate-based microcapsule provide an appropriate osteogenic microenvironment for modular bone tissue formation. Carbohydr Polym. 2021; 277:118807. DOI: 10.1016/j.carbpol.2021.118807. View

15.

Yuan B, Wang L, Zhao R, Yang X, Yang X, Zhu X . A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair. Sci Adv. 2020; 6(50). PMC: 7732183. DOI: 10.1126/sciadv.abc4704. View

16.

Du Y, Guo J, Wang J, Mikos A, Zhang S . Hierarchically designed bone scaffolds: From internal cues to external stimuli. Biomaterials. 2019; 218:119334. PMC: 6663598. DOI: 10.1016/j.biomaterials.2019.119334. View

17.

Shaik I, Dasari B, Shaik A, Doos M, Kolli H, Rana D . Functional Role of Inorganic Trace Elements on Enamel and Dentin Formation: A Review. J Pharm Bioallied Sci. 2022; 13(Suppl 2):S952-S956. PMC: 8686917. DOI: 10.4103/jpbs.jpbs_392_21. View

18.

Ao Y, Zhang E, Liu Y, Yang L, Li J, Wang F . Advanced Hydrogels With Nanoparticle Inclusion for Cartilage Tissue Engineering. Front Bioeng Biotechnol. 2022; 10:951513. PMC: 9277358. DOI: 10.3389/fbioe.2022.951513. View

19.

Ren G, Zhou X, Long R, Xie M, Kankala R, Wang S . Biomedical applications of magnetosomes: State of the art and perspectives. Bioact Mater. 2023; 28:27-49. PMC: 10200801. DOI: 10.1016/j.bioactmat.2023.04.025. View

20.

Doan V, Mondal S, Vo T, Ly C, Vu D, Nguyen V . Fluorescence conjugated nanostructured cobalt-doped hydroxyapatite platform for imaging-guided drug delivery application. Colloids Surf B Biointerfaces. 2022; 214:112458. DOI: 10.1016/j.colsurfb.2022.112458. View