Integrative Use of DNA Barcode and Morphology Reveal High Level of Diversity in the Ornamental Fish on the Lower Amazon Basin

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2025 Jan 8

PMID 39775746

Authors

Elciane Araujo de Freitas

Dayana Batista Dos Santos

Charles Samuel Moraes Ferreira

Carlison Silva-Oliveira

Grazielle Fernanda Evangelista-Gomes

Ivana Barbosa Veneza

Affiliations

Soon will be listed here.

Abstract

The Amazon basin is the world's largest hydrographic basin, in terms of both its total area and its species diversity, with more than 2,700 species of fish. Despite this diversity, the data available on the fish fauna of the Amazon basin are still relatively scant and incomplete, in particular from the streams and floodplain lakes of the lower Amazon, which may contain a large proportion of the still undescribed species of the basin. Many of these species are expected to be of interest to the ornamental fish market. The investigation of the diversity of potential ornamental fish using molecular tools is even more limited. Given this scenario, the present study employed DNA barcoding to investigate the diversity of ornamental fish found in two streams and a floodplain lake of the lower Amazon. The mitochondrially encoded cytochrome c oxidase I (MT-CO1) molecular marker was used to identify the taxa, in combination with morphological keys. A total of 51 ornamental species were identified, representing 13 families and three orders. A majority of the species were found at only one of the sampling points, which indicates that the distribution of the species is influenced by ecological factors. The most speciose order was the Characiformes, followed by the Cichliformes and Siluriformes, while the family with the greatest diversity of species was the Acestrorhamphidae (31.3% of the total number of species), followed by the Cichlidae (27.4%), and the Lebiasinidae (9.8%). One specie was registered in the region of the lower Amazon for the first time, and evidence was found of the possible existence of species not formally described of Aphyocharax, Astyanax, Apareiodon and Hemigrammus.

References

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

Jezequel C, Tedesco P, Bigorne R, Maldonado-Ocampo J, Ortega H, Hidalgo M . A database of freshwater fish species of the Amazon Basin. Sci Data. 2020; 7(1):96. PMC: 7081286. DOI: 10.1038/s41597-020-0436-4. View

Guimaraes-Costa A, Machado F, Oliveira R, Silva-Costa V, Andrade M, Giarrizzo T . Fish diversity of the largest deltaic formation in the Americas - a description of the fish fauna of the Parnaíba Delta using DNA Barcoding. Sci Rep. 2019; 9(1):7530. PMC: 6525184. DOI: 10.1038/s41598-019-43930-z. View

Reis R, Albert J, Di Dario F, Mincarone M, Petry P, Rocha L . Fish biodiversity and conservation in South America. J Fish Biol. 2016; 89(1):12-47. DOI: 10.1111/jfb.13016. View

Tisseuil C, Cornu J, Beauchard O, Brosse S, Darwall W, Holland R . Global diversity patterns and cross-taxa convergence in freshwater systems. J Anim Ecol. 2012; 82(2):365-76. DOI: 10.1111/1365-2656.12018. View

Bogota-Gregory J, Lima F, Correa S, Silva-Oliveira C, Jenkins D, Ribeiro F . Biogeochemical water type influences community composition, species richness, and biomass in megadiverse Amazonian fish assemblages. Sci Rep. 2020; 10(1):15349. PMC: 7501869. DOI: 10.1038/s41598-020-72349-0. View

Hoseinifar S, Maradonna F, Faheem M, Harikrishnan R, Devi G, Ringo E . Sustainable Ornamental Fish Aquaculture: The Implication of Microbial Feed Additives. Animals (Basel). 2023; 13(10). PMC: 10215764. DOI: 10.3390/ani13101583. View

Ward R, Zemlak T, Innes B, Last P, Hebert P . DNA barcoding Australia's fish species. Philos Trans R Soc Lond B Biol Sci. 2005; 360(1462):1847-57. PMC: 1609232. DOI: 10.1098/rstb.2005.1716. View

Hebert P, Cywinska A, Ball S, deWaard J . Biological identifications through DNA barcodes. Proc Biol Sci. 2003; 270(1512):313-21. PMC: 1691236. DOI: 10.1098/rspb.2002.2218. View

10.

Oberdorff T, Dias M, Jezequel C, Albert J, Arantes C, Bigorne R . Unexpected fish diversity gradients in the Amazon basin. Sci Adv. 2019; 5(9):eaav8681. PMC: 6739107. DOI: 10.1126/sciadv.aav8681. View

11.

de Santana C, Crampton W, Dillman C, Frederico R, Sabaj M, Covain R . Unexpected species diversity in electric eels with a description of the strongest living bioelectricity generator. Nat Commun. 2019; 10(1):4000. PMC: 6736962. DOI: 10.1038/s41467-019-11690-z. View

12.

Ali F, Ismail M, Aly W . DNA barcoding to characterize biodiversity of freshwater fishes of Egypt. Mol Biol Rep. 2020; 47(8):5865-5877. DOI: 10.1007/s11033-020-05657-3. View

13.

Silva-Oliveira C, Canto A, Ribeiro F . Bryconops munduruku (Characiformes: Characidae), a new species of fish from the lower Tapajós River basin, Brazil. Zootaxa. 2015; 3994(1):133-41. DOI: 10.11646/zootaxa.3994.1.7. View

14.

Guimaraes K, de Sousa M, Ribeiro F, Porto J, Rodrigues L . DNA barcoding of fish fauna from low order streams of Tapajós River basin. PLoS One. 2018; 13(12):e0209430. PMC: 6303048. DOI: 10.1371/journal.pone.0209430. View

15.

Kimura M . A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2):111-20. DOI: 10.1007/BF01731581. View

16.

Lozano A, Lasso-Alcala O, Bittencourt P, Taphorn D, Perez N, Farias I . A new species of (Teleostei, Cichlidae) from the Orinoco River and Gulf of Paria basins, northern South America. Zookeys. 2023; 1113:111-152. PMC: 9848875. DOI: 10.3897/zookeys.1113.81240. View

17.

Balakrishnan R . Species concepts, species boundaries and species identification: a view from the tropics. Syst Biol. 2005; 54(4):689-93. DOI: 10.1080/10635150590950308. View

18.

Bhattacharya M, Sharma A, Patra B, Sharma G, Seo E, Nam J . DNA barcoding to fishes: current status and future directions. Mitochondrial DNA A DNA Mapp Seq Anal. 2015; 27(4):2744-52. DOI: 10.3109/19401736.2015.1046175. View

19.

Tedesco P, Beauchard O, Bigorne R, Blanchet S, Buisson L, Conti L . A global database on freshwater fish species occurrence in drainage basins. Sci Data. 2017; 4:170141. PMC: 5625552. DOI: 10.1038/sdata.2017.141. View

20.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View