Effect of Conjugative Transfer of Antibiotic Resistance Genes Mediated by Plasmids on the Microecology of Different Intestinal Segments

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2025 Jan 7

PMID 39764443

Authors

Chengshi Ding

Li Yan

Kai Zhang

Xiangxiang Liu

Ziyu Liu

Shaowei Hou

Jing Ma

Zhiping Wu

Hongfei Wei

Affiliations

Soon will be listed here.

Abstract

Introduction: The conjugative transfer of antibiotic resistance genes (ARGs) mediated by plasmids occurred in different intestinal segments of mice was explored.

Methods: The location of ARG donor bacteria and ARGs was investigated by qPCR, flow cytometry, and small animal imaging. The resistant microbiota was analyzed by gene amplification sequencing.

Results: The small intestine was the main site for the location of ARG donor bacteria and ARGs. The intestinal microbiota richness of the small intestine (duodenum and jejunum) and the large intestine (cecum, colon, and rectum) increased, and the ileum microbiota richness decreased under the action of donor bacteria. The differences in the number of bacteria in the small intestine and the large intestine, as well as the relative richness of Firmicutes from the small intestine to the large intestine, decreased. By contrast, the relative abundance of Proteobacteria increased. The intake of resistant plasmids alleviated the impact of antibiotics on intestinal microbiota, particularly increasing the proportion of Proteobacteria and Bacteroides, which were presumed to be susceptible to ARGs.

Discussion: The acquisition of ARGs by intestinal microbes is an important reason why infectious diseases are difficult to cure, which brings risks to human health and intestinal microecology.

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