Advanced Reactivity-based Sequencing Methods for MRNA Epitranscriptome Profiling

Overview

Journal RSC Chem Biol

Publisher Royal Society of Chemistry

Specialty Biology

Date 2025 Jan 6

PMID 39759443

Authors

Zhihe Cai

Peizhe Song

Kemiao Yu

Guifang Jia

Affiliations

Soon will be listed here.

Abstract

Currently, over 170 chemical modifications identified in RNA introduce an additional regulatory attribute to gene expression, known as the epitranscriptome. The development of detection methods to pinpoint the location and quantify these dynamic and reversible modifications has significantly expanded our understanding of their roles. This review goes deep into the latest progress in enzyme- and chemical-assisted sequencing methods, highlighting the opportunities presented by these reactivity-based techniques for detailed characterization of RNA modifications. Our survey provides a deeper understanding of the function and biological roles of RNA modification.

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