Re-arranging the Cis-regulatory Modules of Hox Complex in Drosophila Via FLP-FRT and CRISPR/Cas9
Overview
Overview
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
FLP-FRT, a well-established technique for genome manipulation, and the revolutionary CRISPR/Cas9, known for its targeted indels, are combined in a novel approach. This unique method is applied to the Hox genes in the Drosophila melanogaster bithorax complex, which are closely located to the cis-regulatory modules that define their spatial-temporal regulation. The number and position of these genes are directly correlated to their expression pattern. This chapter unveils the exciting potential of this combinatorial use of FLP-FRT and CRISPR-Cas9 to rearrange the cis-regulatory modules of the Hox complex in Drosophila melanogaster.
References
1.
Li J, Li Y, Pawlik K, Napierala J, Napierala M
. A CRISPR-Cas9, Cre-, and Flp- Cascade Strategy for the Precise and Efficient Integration of Exogenous DNA into Cellular Genomes. CRISPR J. 2020; 3(6):470-486.
PMC: 7757697.
DOI: 10.1089/crispr.2020.0042.
View
2.
Gates C, Cox M
. FLP recombinase is an enzyme. Proc Natl Acad Sci U S A. 1988; 85(13):4628-32.
PMC: 280488.
DOI: 10.1073/pnas.85.13.4628.
View
3.
Golic K, Lindquist S
. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell. 1989; 59(3):499-509.
DOI: 10.1016/0092-8674(89)90033-0.
View