The Use of EDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples
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Abstract
We describe a protocol to amplify DNA barcodes of known and unknown taxa of Phytophthora and related plant pathogenic oomycetes from a range of environments. The methods focus on sampling pathogen propagules from water using in situ sampling and filtration equipment and buffers that enable efficient storage and DNA extraction for later downstream processing.
References
1.
Renshaw M, Olds B, Jerde C, McVeigh M, Lodge D
. The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol-chloroform-isoamyl alcohol DNA extraction. Mol Ecol Resour. 2014; 15(1):168-76.
PMC: 4312482.
DOI: 10.1111/1755-0998.12281.
View
2.
Matsuoka S, Sugiyama Y, Nagano M, Doi H
. Influence of DNA extraction kits on freshwater fungal DNA metabarcoding. PeerJ. 2022; 10:e13477.
PMC: 9150701.
DOI: 10.7717/peerj.13477.
View
3.
Scibetta S, Schena L, Chimento A, Cacciola S, Cooke D
. A molecular method to assess Phytophthora diversity in environmental samples. J Microbiol Methods. 2012; 88(3):356-68.
DOI: 10.1016/j.mimet.2011.12.012.
View