CHD6 Eviction of Promoter Nucleosomes Maintains Housekeeping Transcriptional Program in Prostate Cancer

Overview

Journal Mol Ther Nucleic Acids

Publisher Cell Press

Date 2024 Dec 24

PMID 39717618

Authors

Lina Bu

Shaodong Huang

Ziyan Rao

Chenyang Wu

Bryan-Yu Sun

Yanhua Liu

Lin He

Dongyu Zhao

Affiliations

Soon will be listed here.

Abstract

CHD6, a member of the chromodomain helicase DNA-binding protein family, has been implicated in various diseases and tumors. However, its precise binding model of CHD6 on regulatory functional genes remains poorly understood. In this study, we discovered sharp peaks of CHD6, as the first member of CHD family for housekeeping process, binding only to the promoter region of genes in the C4-2 cell line. These genes, with conserved sharp CHD6 peaks across tumor cells, likely represent housekeeping genes and . Genes with sharp CHD6 peaks exhibit stable and low expression levels, sharing epigenetic features similar to housekeeping genes. Furthermore, this regulatory model also exists in both HEK293 cells and cardiomyocytes. Overall, the results of this study demonstrate that CHD6 binds to the promoter regions of housekeeping genes, regulating their histone modifications, chromatin structure, and gene expression.

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