Isolation and Characterization of Iwanai Valley Virus, a New Tick-borne Nairovirus from Ixodes Ovatus Ticks in Hokkaido, Japan

Overview

Journal Arch Virol

Specialty Microbiology

Date 2024 Dec 21

PMID 39708165

Authors

Ryo Matsumura

Daisuke Kobayashi

Takeo Yamauchi

Eunsil Park

Ayano Nishino

Ken Maeda

Shinji Kasai

Kyo Itoyama

Haruhiko Isawa

Affiliations

Soon will be listed here.

Abstract

Most members of the genus Orthonairovirus, represented by Crimean-Congo hemorrhagic fever virus and Nairobi sheep disease virus, are tick-borne, and some have become a public health concern in recent years. Here, we report the isolation and genetic and biological characterization of a new orthonairovirus, designated as "Iwanai Valley virus" (IWVV), from Ixodes ovatus ticks in Hokkaido, Japan. The amino acid sequence of the viral nucleoprotein (NP) was found to be 34-45% identical to those of known orthonairoviruses. Phylogenetic analysis based on amino acid sequences of the viral NP revealed that IWVV is closely related to human-pathogenic tick-borne orthonairoviruses, including Songling virus, Tacheng tick virus 1, and Yezo virus, which were recently discovered in East Asia. BHK-21 (Syrian hamster kidney), Vero (African green monkey kidney), SW-13 (human adrenal adenocarcinoma), HuH-7 (human hepatocellular carcinoma), and ISE6 (Ixodes scapularis tick embryo) cells were found to be susceptible to IWVV infection. Notably, several human-derived cell lines supported efficient replication of the virus. Our findings provide new insights into the diversity, evolutionary dynamics, and geographical distribution of orthonairoviruses in East Asia.

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