Conformational Changes in and Translocation of Small Proteins: Insights into the Ejection Mechanism of Podophages

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2024 Dec 20

PMID 39704524

Authors

Jing Zheng

Hao Xiao

Hao Pang

Li Wang

Jingdong Song

Wenyuan Chen

Lingpeng Cheng

Hongrong Liu

Affiliations

Soon will be listed here.

Abstract

Importance: Podophage T7 core proteins form an elongated trans-envelope channel for genomic DNA delivery into the host cell. The structures of the core proteins within the mature T7 and assembled in the periplasmic tunnel form in the DNA-ejected T7 have been resolved previously. Here, we resolved the structures of two new structural proteins (gp6.7 and gp7.3) within mature T7 and receptor-induced DNA-ejected T7. The gp6.7 protein participates in the assembly of the core complex within mature T7 and the trans-envelope channel during T7 infection; therefore, gp6.7 is a core protein. Before T7 infection, gp7.3 plays a role in promoting the assembly of the nozzle into the adaptor.

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