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Complete Mitochondrial Genome of and Its Phylogenetic Status Within the Family Cobitidae (Cypriniformes)

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Journal Zookeys
Date 2024 Dec 20
PMID 39703235
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Abstract

In this study, the complete mitochondrial genome of was first determined by the primer walking sequence method. The complete mitochondrial genome was 16,574 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (D-loop). The gene arrangement pattern was identical to that of other teleosts. The overall base composition was 29.9% A, 28.5% T, 25.5% C, and 16.1% G, with an A+T bias of 58.4%. Furthermore, phylogenetic analyses were conducted based on 13 PCGs from the mitochondrial genomes of 18 cobitid species using with three different methods (Neighbor-joining, Maximum likelihood, and Bayesian inference). All methods consistently showed that the four species of the genus form a monophyletic group. This study would provide effective molecular information for the species as well as novel genetic marker for the study of species identification.

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