Visualization of Stress Fiber Formation Induced by Photodynamic Therapy with Porphylipoprotein

Overview

Journal Nanomaterials (Basel)

Date 2024 Dec 17

PMID 39683251

Authors

Atsushi Taninaka

Hiromi Kurokawa

Mayuka Kamiyanagi

Osamu Takeuchi

Hirofumi Matsui

Hidemi Shigekawa

Affiliations

Soon will be listed here.

Abstract

We investigated stress fiber formation induced by photodynamic therapy (PDT) with porphylipoprotein (PLP) by observing actin filaments by super-resolution confocal microscopy and measuring the cellular elastic modulus by atomic force microscopy. We identified different intracellular mechanisms of stress fiber formation between RGM1 epithelial cells, which were derived from rat gastric mucosa, and RGK1 cells, which were cancer-like mutants of RGM1. Our findings show that when PLP is used as a photosensitizer in PDT, it selectively induces necrosis in tumors with minimal impact on the surrounding normal tissues, as it is less likely to cause blood flow obstruction.

References

Shimokawa O, Matsui H, Nagano Y, Kaneko T, Shibahara T, Nakahara A . Neoplastic transformation and induction of H+,K+ -adenosine triphosphatase by N-methyl-N'-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line. In Vitro Cell Dev Biol Anim. 2007; 44(1-2):26-30. DOI: 10.1007/s11626-007-9067-8. View

Cheng W, Nie J, Xu L, Liang C, Peng Y, Liu G . pH-Sensitive Delivery Vehicle Based on Folic Acid-Conjugated Polydopamine-Modified Mesoporous Silica Nanoparticles for Targeted Cancer Therapy. ACS Appl Mater Interfaces. 2017; 9(22):18462-18473. DOI: 10.1021/acsami.7b02457. View

Sanz-Moreno V, Marshall C . The plasticity of cytoskeletal dynamics underlying neoplastic cell migration. Curr Opin Cell Biol. 2010; 22(5):690-6. DOI: 10.1016/j.ceb.2010.08.020. View

Kurokawa H, Taninaka A, Shigekawa H, Matsui H . Dabigatran Etexilate Induces Cytotoxicity in Rat Gastric Epithelial Cell Line via Mitochondrial Reactive Oxygen Species Production. Cells. 2021; 10(10). PMC: 8533938. DOI: 10.3390/cells10102508. View

Blocker A, Griffiths G, Olivo J, Hyman A, Severin F . A role for microtubule dynamics in phagosome movement. J Cell Sci. 1998; 111 ( Pt 3):303-12. DOI: 10.1242/jcs.111.3.303. View

Horiuchi D, Collins C, Bhat P, Barkus R, DiAntonio A, Saxton W . Control of a kinesin-cargo linkage mechanism by JNK pathway kinases. Curr Biol. 2007; 17(15):1313-7. PMC: 2041807. DOI: 10.1016/j.cub.2007.06.062. View

Kurokawa H, Ito H, Matsui H . Porphylipoprotein Accumulation and Porphylipoprotein Photodynamic Therapy Effects Involving Cancer Cell-Specific Cytotoxicity. Int J Mol Sci. 2021; 22(14). PMC: 8305091. DOI: 10.3390/ijms22147306. View

Nihalani D, Wong H, Holzman L . Recruitment of JNK to JIP1 and JNK-dependent JIP1 phosphorylation regulates JNK module dynamics and activation. J Biol Chem. 2003; 278(31):28694-702. DOI: 10.1074/jbc.M304212200. View

Cavin S, Riedel T, Rosskopfova P, Gonzalez M, Baldini G, Zellweger M . Vascular-targeted low dose photodynamic therapy stabilizes tumor vessels by modulating pericyte contractility. Lasers Surg Med. 2019; 51(6):550-561. DOI: 10.1002/lsm.23069. View

10.

Nichols J, Bae Y . EPR: Evidence and fallacy. J Control Release. 2014; 190:451-64. DOI: 10.1016/j.jconrel.2014.03.057. View

11.

Taninaka A, Kurokawa H, Kamiyanagi M, Ochiai T, Arashida Y, Takeuchi O . Polphylipoprotein-induced autophagy mechanism with high performance in photodynamic therapy. Commun Biol. 2023; 6(1):1212. PMC: 10684771. DOI: 10.1038/s42003-023-05598-0. View

12.

Karwicka M, Pucelik B, Gonet M, Elas M, Dabrowski J . Effects of Photodynamic Therapy with Redaporfin on Tumor Oxygenation and Blood Flow in a Lung Cancer Mouse Model. Sci Rep. 2019; 9(1):12655. PMC: 6718604. DOI: 10.1038/s41598-019-49064-6. View

13.

Lovell J, Jin C, Huynh E, Jin H, Kim C, Rubinstein J . Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents. Nat Mater. 2011; 10(4):324-32. DOI: 10.1038/nmat2986. View

14.

Doi M, Tanaka H, Ohoto T, Miura N, Sakurai Y, Hatakeyama H . Reactivation of Anticancer Immunity by Resetting Interorgan Crosstalk in Immune-Suppressive Cells with a Nanoparticulated Anti-Inflammatory Drug. Small. 2023; 19(16):e2205131. DOI: 10.1002/smll.202205131. View

15.

Kamiyanagi M, Taninaka A, Ugajin S, Nagoshi Y, Kurokawa H, Ochiai T . Cell-Level Analysis Visualizing Photodynamic Therapy with Porphylipoprotein and Talaporphyrin Sodium. Int J Mol Sci. 2022; 23(21). PMC: 9655257. DOI: 10.3390/ijms232113140. View

16.

Jiang M, Esteve-Rudd J, Lopes V, Diemer T, Lillo C, Rump A . Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis. J Cell Biol. 2015; 210(4):595-611. PMC: 4539993. DOI: 10.1083/jcb.201410112. View

17.

Wirth A . Rho kinase and hypertension. Biochim Biophys Acta. 2010; 1802(12):1276-84. DOI: 10.1016/j.bbadis.2010.05.002. View

18.

He C, Duan X, Guo N, Chan C, Poon C, Weichselbaum R . Core-shell nanoscale coordination polymers combine chemotherapy and photodynamic therapy to potentiate checkpoint blockade cancer immunotherapy. Nat Commun. 2016; 7:12499. PMC: 4992065. DOI: 10.1038/ncomms12499. View

19.

Dougherty T, Gomer C, Henderson B, Jori G, Kessel D, Korbelik M . Photodynamic therapy. J Natl Cancer Inst. 1998; 90(12):889-905. PMC: 4592754. DOI: 10.1093/jnci/90.12.889. View

20.

Cui L, Lin Q, Jin C, Jiang W, Huang H, Ding L . A PEGylation-Free Biomimetic Porphyrin Nanoplatform for Personalized Cancer Theranostics. ACS Nano. 2015; 9(4):4484-95. DOI: 10.1021/acsnano.5b01077. View