A Chemigenetic Indicator Based on a Synthetic Chelator and a Green Fluorescent Protein for Imaging of Intracellular Sodium Ions

Overview

Journal RSC Chem Biol

Publisher Royal Society of Chemistry

Specialty Biology

Date 2024 Dec 16

PMID 39678364

Authors

Shiori Takeuchi

Shosei Imai

Takuya Terai

Robert E Campbell

Affiliations

Soon will be listed here.

Abstract

A chemigenetic indicator with an affinity suitable for imaging of intracellular sodium ions (Na) in mammalian cells was developed. The indicator, based on a chimera of green fluorescent protein (GFP) and HaloTag labeled with a synthetic crown ether chelator, was produced by a combination of rational design and directed evolution. In mammalian cells the indicator exhibited an approximately 100% increase in excitation ratio when the cells were treated with 20 mM Na and an ionophore.

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