Potentials of Cytokinesis Blocked Micronucleus Assay in Radiation Triage and Biological Dosimetry

Overview

Journal J Genet Eng Biotechnol

Specialty Biotechnology

Date 2024 Dec 14

PMID 39674629

Authors

G Tamizh Selvan

P Venkatachalam

Affiliations

Soon will be listed here.

Abstract

The measurement of micronucleus (MN) in the cytokinesis-block arrested binucleated cells has been extensively used as a biomarker in many radiation biology applications in specific biodosimetry. Following radiation casualties, medical management of exposed individuals begins with triage and biological dosimetry. The cytokinesis blocked micronucleus (CBMN) assay is the alternate for the gold standard dicentric chromosome assay in radiation dose assessment. In recent years, the CBMN assay has become well-validated and emerged as a method of choice for evaluating occupational and accidental exposures scenario. It is feasible due to its cost-effective, simple, and rapid dose assessment rather than a conventional chromosome aberration assay. PubMed search tool was used with keywords of MN, biodosimetry, radiotherapy and restricted to human samples. Since Fenech and Morely developed the assay, it has undergone many technical and technological reforms as a biomarker of various applications. In this review, we have abridged recent developments of the CBMN assay in radiation triage and biodosimetry, focusing on (a) the influence of variables on dose estimation, (b) the importance of baseline frequency and reported dose-response coefficient values among different laboratories, (c) inter-laboratory comparison and (d) its limitations and means to overcome them.

Citing Articles

A Double Fluorescent Staining Method Increases the Sensitivity of the Cytokinesis-Block Micronucleus Assay.

Udroiu I Methods Protoc. 2025; 8(1.

PMID: 39846689 PMC: 11755469. DOI: 10.3390/mps8010003.

References

Reichhold S, Neubauer O, Ehrlich V, Knasmuller S, Wagner K . No acute and persistent DNA damage after an Ironman triathlon. Cancer Epidemiol Biomarkers Prev. 2008; 17(8):1913-9. DOI: 10.1158/1055-9965.EPI-08-0293. View

Pan Y, Ruan J, Gao G, Wu L, Piao C, Liu J . Laboratory Intercomparison of Cytogenetic Dosimetry Among 38 Laboratories in China. Dose Response. 2019; 17(1):1559325819833473. PMC: 6416682. DOI: 10.1177/1559325819833473. View

Harder D . Temperature and the formation of radiation-induced chromosome aberrations. II. The temperature dependence of lesion repair and lesion interaction. Int J Radiat Biol Relat Stud Phys Chem Med. 1986; 49(4):673-81. DOI: 10.1080/09553008514552921. View

Garcia O, Ramalho A, Di Giorgio M, S Mir S, Espinoza M, Manzano J . Intercomparison in cytogenetic dosimetry among five laboratories from Latin America. Mutat Res. 1995; 327(1-2):33-9. DOI: 10.1016/0027-5107(94)00066-e. View

Huang P, Huang B, Weng H, Nakayama K, Morimoto K . Effects of lifestyle on micronuclei frequency in human lymphocytes in Japanese hard-metal workers. Prev Med. 2009; 48(4):383-8. DOI: 10.1016/j.ypmed.2008.12.023. View

Gregoire E, Barquinero J, Gruel G, Benadjaoud M, Martinez J, Beinke C . RENEB Inter-Laboratory comparison 2017: limits and pitfalls of ILCs. Int J Radiat Biol. 2021; 97(7):888-905. DOI: 10.1080/09553002.2021.1928782. View

Doherty A, Hayes J, Fellows M, Kirk S, ODonovan M . A rapid, semi-automated method for scoring micronuclei in mononucleated mouse lymphoma cells. Mutat Res. 2011; 726(1):36-41. DOI: 10.1016/j.mrgentox.2011.08.002. View

Frieauff W, Martus H, Suter W, Elhajouji A . Automatic analysis of the micronucleus test in primary human lymphocytes using image analysis. Mutagenesis. 2012; 28(1):15-23. DOI: 10.1093/mutage/ges047. View

Garty G, Chen Y, Salerno A, Turner H, Zhang J, Lyulko O . The RABIT: a rapid automated biodosimetry tool for radiological triage. Health Phys. 2010; 98(2):209-17. PMC: 2923588. DOI: 10.1097/HP.0b013e3181ab3cb6. View

10.

Roman D, Locher F, Suter W, Cordier A, Bobadilla M . Evaluation of a new procedure for the flow cytometric analysis of in vitro, chemically induced micronuclei in V79 cells. Environ Mol Mutagen. 1999; 32(4):387-96. View

11.

Voisin P, Benderitter M, Claraz M, Chambrette V, Sorokine-Durm I, Delbos M . The cytogenetic dosimetry of recent accidental overexposure. Cell Mol Biol (Noisy-le-grand). 2001; 47(3):557-64. View

12.

Miller S, Ferrarotto C, Vlahovich S, Wilkins R, Boreham D, Dolling J . Canadian Cytogenetic Emergency network (CEN) for biological dosimetry following radiological/nuclear accidents. Int J Radiat Biol. 2007; 83(7):471-7. DOI: 10.1080/09553000701370860. View

13.

Thierens H, Vral A, Barbe M, Aousalah B, de Ridder L . A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay. Mutat Res. 1999; 445(1):105-11. DOI: 10.1016/s1383-5718(99)00134-5. View

14.

Shibai-Ogata A, Kakinuma C, Hioki T, Kasahara T . Evaluation of high-throughput screening for in vitro micronucleus test using fluorescence-based cell imaging. Mutagenesis. 2011; 26(6):709-19. DOI: 10.1093/mutage/ger037. View

15.

Smolewski P, Ruan Q, Vellon L, Darzynkiewicz Z . Micronuclei assay by laser scanning cytometry. Cytometry. 2001; 45(1):19-26. DOI: 10.1002/1097-0320(20010901)45:1<19::aid-cyto1140>3.0.co;2-g. View

16.

Goh V, Fujishima Y, Nakayama R, Takebayashi K, Yoshida M, Kasai K . Manual Scoring with Shortened 48 h Cytokinesis-Block Micronucleus Assay Feasible for Triage in the Event of a Mass-Casualty Radiation Accident. Radiat Res. 2023; 199(4):385-395. DOI: 10.1667/RADE-22-00191.1. View

17.

Ishikawa H, Yamamoto H, Tian Y, Kawano M, Yamauchi T, Yokoyama K . Effects of ALDH2 gene polymorphisms and alcohol-drinking behavior on micronuclei frequency in non-smokers. Mutat Res. 2003; 541(1-2):71-80. DOI: 10.1016/s1383-5718(03)00179-7. View

18.

Le Roux J, Slabbert J, Smit B, Blekkenhorst G . Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy. Strahlenther Onkol. 1998; 174(2):75-81. DOI: 10.1007/BF03038479. View

19.

Fenech M, Kirsch-Volders M, Rossnerova A, Sram R, Romm H, Bolognesi C . HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems. Int J Hyg Environ Health. 2013; 216(5):541-52. DOI: 10.1016/j.ijheh.2013.01.008. View

20.

Wojcik A, Lloyd D, Romm H, Roy L . Biological dosimetry for triage of casualties in a large-scale radiological emergency:capacity of the EU member states. Radiat Prot Dosimetry. 2009; 138(4):397-401. DOI: 10.1093/rpd/ncp279. View