The Transcription Factor FcMYB3 Responds to Co γ-ray Irradiation of Axillary Buds in L. by Activating the Expression of the NADPH Oxidase,

Overview

Journal Front Plant Sci

Date 2024 Dec 11

PMID 39659416

Authors

Miaoyu Song

Ziyu Chen

Wupur Bahayiding

Jinping Li

Huiqin Ma

Ziran Wang

Affiliations

Soon will be listed here.

Abstract

Plant irradiation has been used to induce genetic variation in crop germplasm. However, the underlying mechanisms of plant responses to ionizing radiation stress are still unclear. In plants, reactive oxygen species (ROS) are produced with abiotic stress. Respiratory burst oxidative homologs (Rboh) genes are important regulators of plant ROS stress responses, but little is known of their involvement in the response to ionizing radiation stress. In this study, young branches of L. were irradiated with Co γ-rays and axillary buds were collected after 3- 48 h after irradiation. The differentially expressed genes (DEGs; p< 0.05) detected included an early (6 h) and sustained increase in member of the MAPK signaling pathway. The activities of superoxide dismutase SOD, POD and CAT in fig axillary buds showed a trend of first decrease and then increase with time, while the contents of MDA and H2O2 maintained an overall upward trend. The analysis of differentially expressed genes (DEGs; p < 0.05) indicated an early (6 h) and sustained increase in member of the MAPK signaling pathway. DEGs for glutathione-s-transferase and genes involved in phenylpropanoid and flavonoid biosynthesis pathways were detected at all time points, indicating that γ-irradiation induced an increased capacity for in ROS-scavenging. Substantial changes in the expression of MYB, NAC and bHLH transcription factor family members were also seen to occur within 6 h after irradiation. Taking Rboh-derived ROS signaling pathway as the entry point, the MYB transcription factor, FcMYB3, was identified as an potential upstream regulator of in a yeast one hybrid assay and this interaction verified by LUC and EMSA experiments. The knock-down and overexpression of indicated that FcMYB3 is a positive regulator of ROS accumulation in response to γ-ray radiation stress responses in fig. Our results will provide a better understanding of the mechanisms of radiation tolerance in plant materials.

References

Marteijn J, Lans H, Vermeulen W, Hoeijmakers J . Understanding nucleotide excision repair and its roles in cancer and ageing. Nat Rev Mol Cell Biol. 2014; 15(7):465-81. DOI: 10.1038/nrm3822. View

Benson F, Baumann P, West S . Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature. 1998; 391(6665):401-4. DOI: 10.1038/34937. View

Lempiainen H, Halazonetis T . Emerging common themes in regulation of PIKKs and PI3Ks. EMBO J. 2009; 28(20):3067-73. PMC: 2752028. DOI: 10.1038/emboj.2009.281. View

Liu D, Li Y, Zhou Z, Xiang X, Liu X, Wang J . Tobacco transcription factor bHLH123 improves salt tolerance by activating NADPH oxidase NtRbohE expression. Plant Physiol. 2021; 186(3):1706-1720. PMC: 8260122. DOI: 10.1093/plphys/kiab176. View

Koyama S, Kodama S, Suzuki K, Matsumoto T, Miyazaki T, Watanabe M . Radiation-induced long-lived radicals which cause mutation and transformation. Mutat Res. 1998; 421(1):45-54. DOI: 10.1016/s0027-5107(98)00153-5. View

Wang L, Chen H, Wang C, Hu Z, Yan S . Negative regulator of E2F transcription factors links cell cycle checkpoint and DNA damage repair. Proc Natl Acad Sci U S A. 2018; 115(16):E3837-E3845. PMC: 5910849. DOI: 10.1073/pnas.1720094115. View

Qi F, Li J, Ai Y, Shangguan K, Li P, Lin F . DGK5β-derived phosphatidic acid regulates ROS production in plant immunity by stabilizing NADPH oxidase. Cell Host Microbe. 2024; 32(3):425-440.e7. DOI: 10.1016/j.chom.2024.01.011. View

Bleuyard J, Gallego M, Savigny F, White C . Differing requirements for the Arabidopsis Rad51 paralogs in meiosis and DNA repair. Plant J. 2005; 41(4):533-45. DOI: 10.1111/j.1365-313X.2004.02318.x. View

Vranova E, Atichartpongkul S, Villarroel R, Van Montagu M, Inze D, Van Camp W . Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress. Proc Natl Acad Sci U S A. 2002; 99(16):10870-5. PMC: 125065. DOI: 10.1073/pnas.152337999. View

10.

Caplin N, Willey N . Ionizing Radiation, Higher Plants, and Radioprotection: From Acute High Doses to Chronic Low Doses. Front Plant Sci. 2018; 9:847. PMC: 6028737. DOI: 10.3389/fpls.2018.00847. View

11.

Zhang H, Gu Z, Wu Q, Yang L, Liu C, Ma H . Arabidopsis PARG1 is the key factor promoting cell survival among the enzymes regulating post-translational poly(ADP-ribosyl)ation. Sci Rep. 2015; 5:15892. PMC: 4626836. DOI: 10.1038/srep15892. View

12.

Moon H, Lee B, Choi G, Shin D, Prasad D, Lee O . NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci U S A. 2002; 100(1):358-63. PMC: 140977. DOI: 10.1073/pnas.252641899. View

13.

Neill S, Desikan R, Hancock J . Hydrogen peroxide signalling. Curr Opin Plant Biol. 2002; 5(5):388-95. DOI: 10.1016/s1369-5266(02)00282-0. View

14.

Lord C, Ashworth A . The DNA damage response and cancer therapy. Nature. 2012; 481(7381):287-94. DOI: 10.1038/nature10760. View

15.

Mori K, Shirasawa K, Nogata H, Hirata C, Tashiro K, Habu T . Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.). Sci Rep. 2017; 7:41124. PMC: 5264649. DOI: 10.1038/srep41124. View

16.

Boller T, He S . Innate immunity in plants: an arms race between pattern recognition receptors in plants and effectors in microbial pathogens. Science. 2009; 324(5928):742-4. PMC: 2729760. DOI: 10.1126/science.1171647. View

17.

Hafsi C, Collado-Arenal A, Wang H, Sanz-Fernandez M, Sahrawy M, Shabala S . The role of NADPH oxidases in regulating leaf gas exchange and ion homeostasis in Arabidopsis plants under cadmium stress. J Hazard Mater. 2022; 429:128217. DOI: 10.1016/j.jhazmat.2022.128217. View

18.

Calucci L, Pinzino C, Zandomeneghi M, Capocchi A, Ghiringhelli S, Saviozzi F . Effects of gamma-irradiation on the free radical and antioxidant contents in nine aromatic herbs and spices. J Agric Food Chem. 2003; 51(4):927-34. DOI: 10.1021/jf020739n. View

19.

Desikan R, Hancock J, Neill S . Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol. 2001; 127(1):159-72. PMC: 117972. DOI: 10.1104/pp.127.1.159. View

20.

Turkan I, Uzilday B, Dietz K, Brautigam A, Ozgur R . Reactive oxygen species and redox regulation in mesophyll and bundle sheath cells of C4 plants. J Exp Bot. 2018; 69(14):3321-3331. DOI: 10.1093/jxb/ery064. View