Synthesis, Characterization, and Biomedical Applications of Bacteriocin-Selenium Nanoconjugates

Overview

Journal Probiotics Antimicrob Proteins

Publisher Springer

Specialties Infectious Diseases
Microbiology
Nutritional Sciences
Pharmacology

Date 2024 Dec 10

PMID 39658757

Authors

Sana M H Al-Shimmary

Amina N Al-Thwani

Affiliations

Soon will be listed here.

Abstract

The antibiotic overuse in hospitals, the food industry, and animal feed over past times has led to a significant rise in the incidence of antibiotic-resistant bacteria. To address these potentially life-threatening antibiotic-resistant illnesses, a quick identification and development of novel antimicrobials are necessary. The aim of this study was to synthesize a novel bacteriocin-nanoconjugates by combining selenium nanoparticles with purified bacteriocin from the Enterococcus faecium SMAA23 and investigate some of its biomedical activities. The nanoconjugates were characterized via X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray desorption (EDX), and zeta potential analytical techniques. There is investigation of the antibacterial, antifungal, and anticancer properties of nanoconjugates. Purified bacteriocin has a known molecular weight of approximately 43,000 Daltons. The characterization of nanoparticles and nanoconjugates was performed. The crystallite size of nanoconjugate was determined via X-ray diffraction (XRD) to be 15.29 nm. Transmission electron microscopy (TEM) detected particles of irregular form of nanoconjugate, measuring between 11 and 24 nm in diameter. Energy dispersive X-ray spectroscopy (EDX) confirmed the presence of selenium and protein. The measured zeta potential was - 12.1 + 0.12 mV. The results revealed potent antibacterial activity against Acinetobacter baumannii, with a growth inhibition zone of 23 mm ± SD. A minimum inhibitory concentration (MIC) of nanoconjugate was 15.625 µg/mL, while a minimum bactericidal concentration (MBC) was 31.25 µg/mL. The application of scanning electron microscopy (SEM) enhanced the rupture of the bacterial cell wall. The antifungal activity against C. albicans and C. tropicalis resulted in growth inhibition zones of 14 mm and 16 mm (± SD), respectively. Various concentrations of the nanoconjugate strongly inhibited MDA-MB-231 cells in the MTT experiment. The novel synthesized bacteriocin-nanoconjugates exhibited substantial antibacterial, antifungal, and anticancer properties.

References

Soltani S, Hammami R, Cotter P, Rebuffat S, Ben Said L, Gaudreau H . Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations. FEMS Microbiol Rev. 2020; 45(1). PMC: 7794045. DOI: 10.1093/femsre/fuaa039. View

Duquesne S, Destoumieux-Garzon D, Peduzzi J, Rebuffat S . Microcins, gene-encoded antibacterial peptides from enterobacteria. Nat Prod Rep. 2007; 24(4):708-34. DOI: 10.1039/b516237h. View

Sim S, Wong N . Nanotechnology and its use in imaging and drug delivery (Review). Biomed Rep. 2021; 14(5):42. PMC: 7953199. DOI: 10.3892/br.2021.1418. View

Sidhu P, Nehra K . Bacteriocin-capped silver nanoparticles for enhanced antimicrobial efficacy against food pathogens. IET Nanobiotechnol. 2020; 14(3):245-252. PMC: 8676405. DOI: 10.1049/iet-nbt.2019.0323. View

Amer S, AbuShady H, Refay R, Mailam M . Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles. J Genet Eng Biotechnol. 2021; 19(1):13. PMC: 7817718. DOI: 10.1186/s43141-020-00093-z. View

Naskar A, Kim K . Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins. Pharmaceutics. 2021; 13(1). PMC: 7826801. DOI: 10.3390/pharmaceutics13010086. View

Lee E, Khan I, Oh D . Evaluation of the efficacy of nisin-loaded chitosan nanoparticles against foodborne pathogens in orange juice. J Food Sci Technol. 2018; 55(3):1127-1133. PMC: 5821672. DOI: 10.1007/s13197-017-3028-3. View

Qiao X, Du R, Wang Y, Han Y, Zhou Z . Purification, characterization and mode of action of enterocin, a novel bacteriocin produced by Enterococcus faecium TJUQ1. Int J Biol Macromol. 2019; 144:151-159. DOI: 10.1016/j.ijbiomac.2019.12.090. View

Vahdati M, Moghadam T . Synthesis and Characterization of Selenium Nanoparticles-Lysozyme Nanohybrid System with Synergistic Antibacterial Properties. Sci Rep. 2020; 10(1):510. PMC: 6965607. DOI: 10.1038/s41598-019-57333-7. View

10.

Awadelkareem A, Al-Shammari E, Elkhalifa A, Adnan M, Siddiqui A, Patel M . Biosynthesized Silver Nanoparticles from Miller Leaf Extract Exhibits Antibacterial, Antioxidant, Anti-Quorum-Sensing, Antibiofilm, and Anti-Metastatic Activities. Antibiotics (Basel). 2022; 11(7). PMC: 9311509. DOI: 10.3390/antibiotics11070853. View

11.

Yuan Q, Xiao R, Afolabi M, Bomma M, Xiao Z . Evaluation of Antibacterial Activity of Selenium Nanoparticles against Food-Borne Pathogens. Microorganisms. 2023; 11(6). PMC: 10300849. DOI: 10.3390/microorganisms11061519. View

12.

Patel M, Siddiqui A, Hamadou W, Surti M, Awadelkareem A, Ashraf S . Inhibition of Bacterial Adhesion and Antibiofilm Activities of a Glycolipid Biosurfactant from with Its Physicochemical and Functional Properties. Antibiotics (Basel). 2021; 10(12). PMC: 8698754. DOI: 10.3390/antibiotics10121546. View

13.

Elshikh M, Ahmed S, Funston S, Dunlop P, McGaw M, Marchant R . Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnol Lett. 2016; 38(6):1015-9. PMC: 4853446. DOI: 10.1007/s10529-016-2079-2. View

14.

Nag S, Kar S, Mishra S, Stany B, Seelan A, Mohanto S . Unveiling Green Synthesis and Biomedical Theranostic paradigms of Selenium Nanoparticles (SeNPs) - A state-of-the-art comprehensive update. Int J Pharm. 2024; 662:124535. DOI: 10.1016/j.ijpharm.2024.124535. View

15.

El-Sherbiny G, Kalaba M, Foda A, M E S, Youssef A, Elsehemy I . Nanoemulsion of cinnamon oil to combat colistin-resistant Klebsiella pneumoniae and cancer cells. Microb Pathog. 2024; 192:106705. DOI: 10.1016/j.micpath.2024.106705. View

16.

Abdel-Fatah S, El-Sherbiny G, Khalaf M, Baz A, El-Sayed A, El-Batal A . Boosting the Anticancer Activity of Aspergillus flavus "endophyte of Jojoba" Taxol via Conjugation with Gold Nanoparticles Mediated by γ-Irradiation. Appl Biochem Biotechnol. 2022; 194(8):3558-3581. PMC: 9270289. DOI: 10.1007/s12010-022-03906-8. View

17.

Shakhatreh M, Al-Smadi M, Khabour O, Shuaibu F, Hussein E, Alzoubi K . Study of the antibacterial and antifungal activities of synthetic benzyl bromides, ketones, and corresponding chalcone derivatives. Drug Des Devel Ther. 2016; 10:3653-3660. PMC: 5108482. DOI: 10.2147/DDDT.S116312. View

18.

Kalaba M, El-Sherbiny G, Ewais E, Darwesh O, Moghannem S . Green synthesis of zinc oxide nanoparticles (ZnO-NPs) by Streptomyces baarnensis and its active metabolite (Ka): a promising combination against multidrug-resistant ESKAPE pathogens and cytotoxicity. BMC Microbiol. 2024; 24(1):254. PMC: 11232237. DOI: 10.1186/s12866-024-03392-4. View

19.

Rochin-Medina J, Ramirez-Medina H, Rangel-Peraza J, Pineda-Hidalgo K, Iribe-Arellano P . Use of whey as a culture medium for Bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity. Food Sci Technol Int. 2017; 24(1):35-42. DOI: 10.1177/1082013217724705. View

20.

Bradshaw J . Cationic antimicrobial peptides : issues for potential clinical use. BioDrugs. 2003; 17(4):233-40. DOI: 10.2165/00063030-200317040-00002. View