Comparing Lactic Acid Bacteria Biodiversity in Irritable Bowel Syndrome and Healthy Gut Microbiota

Overview

Journal Food Sci Nutr

Specialty Biotechnology

Date 2024 Dec 2

PMID 39619992

Authors

Aysooda Azimi

Masoud Yavarmanesh

Mehran Gholamin

Affiliations

Soon will be listed here.

Abstract

Irritable bowel syndrome (IBS) is a prevalent gut disorder linked to changes in the gut microbiota, including lactic acid bacteria (LAB). However, research on LAB biodiversity in IBS patients is limited. This study aimed to compare LAB microbiota in healthy individuals and those with IBS through biochemical and molecular techniques. Fecal samples from 15 IBS patients and 13 healthy individuals were collected, and LAB were isolated using biochemical methods. Fifty isolates were chosen based on Gram staining and catalase tests and identified through 16S rRNA gene sequencing. A phylogenetic tree was used to analyze strain diversity, and correlation diagrams and swarm plots were employed to explore variable relationships. The study revealed a significant difference in LAB numbers between IBS and healthy subjects, with average of 5.91 and 6.63, respectively. Most bacteria were Gram-positive cocci or bacilli, with homofermentative characteristics, except for one heterofermentative sample from the healthy group. Both IBS and healthy groups exhibited strains from and genera, with being predominant in both. Demographic analysis showed higher IBS prevalence among individuals aged 20-40, with IBS-C more common in women and IBS-D in men. The study concluded that individuals with IBS had significantly lower LAB microbiota counts, potentially impacting intestinal defense function. Further exploration of LAB behavioral and immunomodulatory traits may enhance understanding of intestinal microbiota's role in IBS and aid in developing treatment strategies.

References

Kosako M, Akiho H, Miwa H, Kanazawa M, Fukudo S . Impact of symptoms by gender and age in Japanese subjects with irritable bowel syndrome with constipation (IBS-C): a large population-based internet survey. Biopsychosoc Med. 2018; 12:12. PMC: 6122187. DOI: 10.1186/s13030-018-0131-2. View

Ni K, Wang Y, Li D, Cai Y, Pang H . Characterization, identification and application of lactic Acid bacteria isolated from forage paddy rice silage. PLoS One. 2015; 10(3):e0121967. PMC: 4372580. DOI: 10.1371/journal.pone.0121967. View

Soltan Dallal M, Zamaniahari S, Davoodabadi A, Hosseini M, Rajabi Z . Identification and characterization of probiotic lactic acid bacteria isolated from traditional persian pickled vegetables. GMS Hyg Infect Control. 2017; 12:Doc15. PMC: 5627144. DOI: 10.3205/dgkh000300. View

Fadgyas-Stanculete M, Buga A, Popa-Wagner A, Dumitrascu D . The relationship between irritable bowel syndrome and psychiatric disorders: from molecular changes to clinical manifestations. J Mol Psychiatry. 2014; 2(1):4. PMC: 4223878. DOI: 10.1186/2049-9256-2-4. View

Rodino-Janeiro B, Vicario M, Alonso-Cotoner C, Pascua-Garcia R, Santos J . A Review of Microbiota and Irritable Bowel Syndrome: Future in Therapies. Adv Ther. 2018; 35(3):289-310. PMC: 5859043. DOI: 10.1007/s12325-018-0673-5. View

Adimpong D, Nielsen D, Sorensen K, Derkx P, Jespersen L . Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products. BMC Microbiol. 2012; 12:75. PMC: 3463448. DOI: 10.1186/1471-2180-12-75. View

Tamura K, Stecher G, Kumar S . MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 2021; 38(7):3022-3027. PMC: 8233496. DOI: 10.1093/molbev/msab120. View

Liu H, Wu H, Chen Y, Chen Y, Shen X, Liu T . Altered molecular signature of intestinal microbiota in irritable bowel syndrome patients compared with healthy controls: A systematic review and meta-analysis. Dig Liver Dis. 2017; 49(4):331-337. DOI: 10.1016/j.dld.2017.01.142. View

Moraes-Filho J, Quigley E . THE INTESTINAL MICROBIOTA AND THE ROLE OF PROBIOTICS IN IRRITABLE BOWEL SYNDROME: a review. Arq Gastroenterol. 2016; 52(4):331-8. DOI: 10.1590/S0004-28032015000400015. View

10.

Lacy B, Patel N . Rome Criteria and a Diagnostic Approach to Irritable Bowel Syndrome. J Clin Med. 2017; 6(11). PMC: 5704116. DOI: 10.3390/jcm6110099. View

11.

El-Salhy M, Gundersen D, Gilja O, Hatlebakk J, Hausken T . Is irritable bowel syndrome an organic disorder?. World J Gastroenterol. 2014; 20(2):384-400. PMC: 3923014. DOI: 10.3748/wjg.v20.i2.384. View

12.

Camilleri M . Diagnosis and Treatment of Irritable Bowel Syndrome: A Review. JAMA. 2021; 325(9):865-877. DOI: 10.1001/jama.2020.22532. View

13.

Kassinen A, Krogius-Kurikka L, Makivuokko H, Rinttila T, Paulin L, Corander J . The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterology. 2007; 133(1):24-33. DOI: 10.1053/j.gastro.2007.04.005. View

14.

Wang X, Wang W, Lv H, Zhang H, Liu Y, Zhang M . Probiotic Potential and Wide-spectrum Antimicrobial Activity of Lactic Acid Bacteria Isolated from Infant Feces. Probiotics Antimicrob Proteins. 2020; 13(1):90-101. DOI: 10.1007/s12602-020-09658-3. View

15.

Carroll I, Ringel-Kulka T, Keku T, Chang Y, Packey C, Sartor R . Molecular analysis of the luminal- and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome. Am J Physiol Gastrointest Liver Physiol. 2011; 301(5):G799-807. PMC: 3220325. DOI: 10.1152/ajpgi.00154.2011. View

16.

Zhuang X, Tian Z, Li L, Zeng Z, Chen M, Xiong L . Fecal Microbiota Alterations Associated With Diarrhea-Predominant Irritable Bowel Syndrome. Front Microbiol. 2018; 9:1600. PMC: 6068233. DOI: 10.3389/fmicb.2018.01600. View

17.

Kim Y, Kim N . Sex-Gender Differences in Irritable Bowel Syndrome. J Neurogastroenterol Motil. 2018; 24(4):544-558. PMC: 6175559. DOI: 10.5056/jnm18082. View

18.

Herndon C, Wang Y, Lu C . Targeting the gut microbiota for the treatment of irritable bowel syndrome. Kaohsiung J Med Sci. 2019; 36(3):160-170. DOI: 10.1002/kjm2.12154. View

19.

Noor S, Ridgway K, Scovell L, Kemsley E, Lund E, Jamieson C . Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota. BMC Gastroenterol. 2010; 10:134. PMC: 3002299. DOI: 10.1186/1471-230X-10-134. View

20.

Ford A, Harris L, Lacy B, Quigley E, Moayyedi P . Systematic review with meta-analysis: the efficacy of prebiotics, probiotics, synbiotics and antibiotics in irritable bowel syndrome. Aliment Pharmacol Ther. 2018; 48(10):1044-1060. DOI: 10.1111/apt.15001. View