The RDNA of C. Elegans: Sequence and Structure

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Mar 11

PMID 3960722

Citations 67

Authors

R E Ellis

J E Sulston

A R Coulson

Affiliations

Soon will be listed here.

Abstract

We have sequenced one complete rDNA tandem repeat from the nematode C. elegans. By comparative analysis we derive secondary structures for the 18s, 5.8s, and 26s rRNA molecules, and comment on other important features of the sequence. We also present the sequence of a junction between the rDNA and non-ribosomal DNA. Finally, we use our data to quantify the evolutionary relationships among several organisms currently studied in developmental biology.

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References

Sulston J, Brenner S . The DNA of Caenorhabditis elegans. Genetics. 1974; 77(1):95-104. PMC: 1213121. DOI: 10.1093/genetics/77.1.95. View

Staden R . Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res. 1982; 10(15):4731-51. PMC: 321125. DOI: 10.1093/nar/10.15.4731. View

Smith G . Evolution of repeated DNA sequences by unequal crossover. Science. 1976; 191(4227):528-35. DOI: 10.1126/science.1251186. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Szostak J, Wu R . Insertion of a genetic marker into the ribosomal DNA of yeast. Plasmid. 1979; 2(4):536-54. DOI: 10.1016/0147-619x(79)90053-2. View

Szostak J, Wu R . Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature. 1980; 284(5755):426-30. DOI: 10.1038/284426a0. View

Petes T . Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell. 1980; 19(3):765-74. DOI: 10.1016/s0092-8674(80)80052-3. View

Winter G, Fields S . Cloning of influenza cDNA ino M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein. Nucleic Acids Res. 1980; 8(9):1965-74. PMC: 324050. DOI: 10.1093/nar/8.9.1965. View

Michot B, Bachellerie J, Raynal F . Sequence and secondary structure of mouse 28S rRNA 5'terminal domain. Organisation of the 5.8S-28S rRNA complex. Nucleic Acids Res. 1982; 10(17):5273-83. PMC: 320870. DOI: 10.1093/nar/10.17.5273. View

10.

Reddy R, Rothblum L, SUBRAHMANYAM C, Liu M, Henning D, Cassidy B . The nucleotide sequence of 8 S RNA bound to preribosomal RNA of Novikoff hepatoma. The 5'-end of 8 S RNA is 5.8 S RNA. J Biol Chem. 1983; 258(1):584-9. View

11.

Kohorn B, Rae P . Nontranscribed spacer sequences promote in vitro transcription of Drosophila ribosomal DNA. Nucleic Acids Res. 1982; 10(21):6879-86. PMC: 326971. DOI: 10.1093/nar/10.21.6879. View

12.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

13.

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

14.

Harrington C, Chikaraishi D . Identification and sequence of the initiation site for rat 45S ribosomal RNA synthesis. Nucleic Acids Res. 1983; 11(10):3317-32. PMC: 325965. DOI: 10.1093/nar/11.10.3317. View

15.

Michot B, Bachellerie J, Raynal F . Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA. Nucleic Acids Res. 1983; 11(10):3375-91. PMC: 325970. DOI: 10.1093/nar/11.10.3375. View

16.

Otsuka T, Nomiyama H, Yoshida H, Kukita T, Kuhara S, Sakaki Y . Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution. Proc Natl Acad Sci U S A. 1983; 80(11):3163-7. PMC: 394000. DOI: 10.1073/pnas.80.11.3163. View

17.

Deininger P . Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Anal Biochem. 1983; 129(1):216-23. DOI: 10.1016/0003-2697(83)90072-6. View

18.

Biggin M, Gibson T, Hong G . Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983; 80(13):3963-5. PMC: 394179. DOI: 10.1073/pnas.80.13.3963. View

19.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

20.

Karn J, Brenner S, Barnett L . New bacteriophage lambda vectors with positive selection for cloned inserts. Methods Enzymol. 1983; 101:3-19. DOI: 10.1016/0076-6879(83)01004-6. View