Whole-Genome Sequence and Characterization of MLY102 Isolated from Infected Tobacco Stalks

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2024 Nov 27

PMID 39596673

Authors

Guan Lin

Juntao Gao

Junxian Zou

Denghui Li

Yu Cui

Yong Liu

Lingxue Kong

Shiwang Liu

Affiliations

Soon will be listed here.

Abstract

Background/objectives: Bacterial wilt disease is a soil-borne disease caused by that causes huge losses to crop economies worldwide.

Methods: In this work, strain MLY102 was isolated and further identified as from a diseased tobacco stalk. The genomic properties of MLY102 were explored by performing biochemical characterization, genome sequencing, compositional analysis, functional annotation and comparative genomic analysis.

Results: MLY102 had a pinkish-red color in the center of the colony surrounded by a milky-white liquid with fluidity on TTC medium. The biochemical results revealed that MLY102 can utilize carbon sources, including D-glucose (dGLU), cane sugar (SAC) and D-trehalose dihydrate (dTRE). Genome sequencing through the DNBSEQ and PacBio platforms revealed a genome size of 5.72 Mb with a G+C content of 67.59%. The genome consists of a circular chromosome and a circular giant plasmid with 5283 protein-coding genes. A comparison of the genomes revealed that MLY102 is closely related to GMI1000 and CMR15 but has 498 special genes and 13 homologous genes in the species-specific gene family, indicating a high degree of genomic uniqueness.

Conclusions: The unique characteristics and genomic data of MLY102 can provide important reference values for the prevention and control of bacterial wilt disease.

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