Genome-Wide Identification of Gene Family in and Expression Pattern Analysis of ClDELLA Protein Under Abiotic Stresses

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Nov 27

PMID 39596328

Authors

Yi Luo

Mengshuang Jin

Junjie Yang

Ye Yang

Runxin Guo

Huan Luo

Tianhao Guo

Jin Xu

Affiliations

Soon will be listed here.

Abstract

The Chinese fir ( is a significant species utilized in afforestation efforts in southern China. It is distinguished by its rapid growth and adaptability to diverse environmental conditions. The GRAS gene family comprises a group of plant-specific transcription factors that play a pivotal role in plant growth and development, response to adversity, and hormone regulatory networks. However, the exploration of the GRAS family in gymnosperm Chinese fir has not yet begun. In this study, a total of 43 genes were identified in the whole genome of Chinese fir, and a phylogenetic analysis classified them into nine distinct subfamilies. Gene structure analysis revealed that the majority of genes lacked introns. It is notable that among these proteins, both and possess distinctive DELLA structural domains. Cis-acting element analysis revealed that nearly all genes contained light-responsive elements, while hormone-responsive elements, environmental-responsive elements (low-temperature- or defense-responsive elements), and meristem-organization-related elements were also identified. Based on transcriptome data and RT-qPCR expression patterns, we analyzed the expression of and genes across different developmental stages, hormones, and three abiotic stresses. Subcellular localization analysis demonstrated that and were localized to the nucleus. Transcriptional activation assays showed that both genes have self-activating activity. In conclusion, the results of this study indicate that the gene family is involved in the response of Chinese fir to environmental stress. Further research on the genes provides valuable information for exploring the potential regulatory network of DELLA proteins in Chinese fir.

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