Comprehensive Analysis of the GRAS Gene Family in and the Response of DELLA Proteins to Paulownia Witches' Broom

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Feb 24

PMID 38397102

Authors

Yixiao Li

Yabing Cao

Yujie Fan

Guoqiang Fan

Affiliations

Soon will be listed here.

Abstract

The (GAI\RGA\SCL) gene family encodes plant-specific transcription factors that play crucial roles in plant growth and development, stress tolerance, and hormone network regulation. Plant dwarfing symptom is mainly regulated by DELLA proteins of the gene subfamily. In this study, the association between the gene family and Paulownia witches' broom (PaWB) was investigated. A total of 79 genes were identified using bioinformatics methods and categorized into 11 groups based on amino acid sequences. Tandem duplication and fragment duplication were found to be the main modes of amplification of the gene family. Gene structure analysis showed that more than 72.1% of the had no introns. The genes also contained unique DELLA structural domains; only , which showed significant response to PaWB phytoplasma infection in stems, showed significant tissue specificity and responded to gibberellin (GA3) in PaWB-infected plants. We found that the internodes were significantly elongated under 100 µmol·L GA3 treatment for 30 days. The subcellular localization analysis indicated that is located in the nucleus and cell membrane. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays confirmed that PfGRAS12 interacted with PfJAZ3 in the nucleus. Our results will lay a foundation for further research on the functions of the gene family and for genetic improvement and breeding of PaWB-resistant trees.

Citing Articles

Genome-Wide Identification of Gene Family in and Expression Pattern Analysis of ClDELLA Protein Under Abiotic Stresses.

Luo Y, Jin M, Yang J, Yang Y, Guo R, Luo H Int J Mol Sci. 2024; 25(22).

PMID: 39596328 PMC: 11594883. DOI: 10.3390/ijms252212262.

Paulownia Witches' Broom Disease: A Comprehensive Review.

Zhang Y, Qiao Z, Li J, Bertaccini A Microorganisms. 2024; 12(5).

PMID: 38792713 PMC: 11123829. DOI: 10.3390/microorganisms12050885.

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