The Impact of Green Tea Kombucha on the Intestinal Health, Gut Microbiota, and Serum Metabolome of Individuals with Excess Body Weight in a Weight Loss Intervention: A Randomized Controlled Trial

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Nov 27

PMID 39594049

Authors

Gabriela Macedo Fraiz

Dandara Baia Bonifacio

Udielle Vermelho Lacerda

Rodrigo Rezende Cardoso

Viviana Corich

Alessio Giacomini

Hercia Stampini Duarte Martino

Sergio Esteban-Echeverria

Ana Romo-Hualde

David Munoz-Prieto

Frederico Augusto Ribeiro de Barros

Fermin I Milagro

Josefina Bressan

Affiliations

Soon will be listed here.

Abstract

Green tea kombucha (GTK) has emerged as a promising probiotic fermented beverage. Few studies have investigated its effect on human health, mainly focusing on intestinal health, microbiota composition, and metabolomics. The present study is a pioneer in investigating the effect of GTK consumption in individuals with excess body weight. This is a randomized controlled trial, lasting ten weeks, with two groups placed under an energy-restricted diet: control (CG, = 29), kombucha (KG, = 30; 200 mL/d). Biological samples and questionnaires were collected before and after the intervention. Microbiota analysis used an amplification of the V4 region of 16S rRNA. Serum untargeted metabolomics used HPLC-TOF mass spectrometry. Intestinal permeability considered the urine excretion of lactulose and mannitol, plasma zonulin, and LPS-binding protein. After the intervention, no differences related to intestinal permeability and microbiota were found between groups, but only the CG had increased fecal pH, lactulose/mannitol ratio, and zonulin. In addition to this, the KG reported lower gastrointestinal symptoms related to motility compared to the CG, and discriminant metabolites (e.g., diethyl malonate) were found strictly in the KG. GTK did not significantly improve gut microbiota and intestinal permeability. However, GTK ameliorated gastrointestinal symptoms and positively influenced the serum metabolome, which may contribute to enhancing the metabolic health of individuals with excess body weight.

References

Cuevas-Sierra A, Romo-Hualde A, Aranaz P, Goni L, Cuervo M, Martinez J . Diet- and sex-related changes of gut microbiota composition and functional profiles after 4 months of weight loss intervention. Eur J Nutr. 2021; 60(6):3279-3301. DOI: 10.1007/s00394-021-02508-0. View

Wang Y, Li T, Liu Y, Yang C, Liu L, Zhang X . Heimao tea polysaccharides ameliorate obesity by enhancing gut microbiota-dependent adipocytes thermogenesis in mice fed with high fat diet. Food Funct. 2022; 13(24):13014-13027. DOI: 10.1039/d2fo02415b. View

Antolak H, Piechota D, Kucharska A . Kombucha Tea-A Double Power of Bioactive Compounds from Tea and Symbiotic Culture of Bacteria and Yeasts (SCOBY). Antioxidants (Basel). 2021; 10(10). PMC: 8532973. DOI: 10.3390/antiox10101541. View

Martinez A, Azevedo G . The Bristol Stool Form Scale: its translation to Portuguese, cultural adaptation and validation. Rev Lat Am Enfermagem. 2012; 20(3):583-9. DOI: 10.1590/s0104-11692012000300021. View

Dey P, Sasaki G, Wei P, Li J, Wang L, Zhu J . Green tea extract prevents obesity in male mice by alleviating gut dysbiosis in association with improved intestinal barrier function that limits endotoxin translocation and adipose inflammation. J Nutr Biochem. 2019; 67:78-89. DOI: 10.1016/j.jnutbio.2019.01.017. View

Wang G, Yuan Y, Li Z, Zhu J, She Z, Chen Y . Cytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus sp. QYM-13. Mar Drugs. 2023; 21(12). PMC: 10745014. DOI: 10.3390/md21120631. View

Na J, Kim E, Na C, Kim S . Citric Acid-Enriched Extract of Ripe (Siebold) Siebold & Zucc. Induces Laxative Effects by Regulating the Expression of Aquaporin 3 and Prostaglandin E in Rats with Loperamide-Induced Constipation. J Med Food. 2022; 25(1):12-23. PMC: 8787707. DOI: 10.1089/jmf.2021.K.0138. View

Prajapati K, Prajapati J, Patel D, Patel R, Varshnei A, Saraf M . Multidisciplinary advances in kombucha fermentation, health efficacy, and market evolution. Arch Microbiol. 2024; 206(9):366. DOI: 10.1007/s00203-024-04086-1. View

Costa M, Vilela D, Fraiz G, Lopes I, Coelho A, Castro L . Effect of kombucha intake on the gut microbiota and obesity-related comorbidities: A systematic review. Crit Rev Food Sci Nutr. 2021; 63(19):3851-3866. DOI: 10.1080/10408398.2021.1995321. View

10.

Costa M, Moreira L, da Silva Duarte V, Cardoso R, Jose V, da Silva B . Kombuchas from Green and Black Tea Modulate the Gut Microbiota and Improve the Intestinal Health of Wistar Rats Fed a High-Fat High-Fructose Diet. Nutrients. 2022; 14(24). PMC: 9787585. DOI: 10.3390/nu14245234. View

11.

Massoud R, Jafari R, Khosravi-Darani K . Kombucha as a Health-Beneficial Drink for Human Health. Plant Foods Hum Nutr. 2024; 79(2):251-259. DOI: 10.1007/s11130-024-01169-8. View

12.

Diez-Ozaeta I, Astiazaran O . Fermented foods: An update on evidence-based health benefits and future perspectives. Food Res Int. 2022; 156:111133. DOI: 10.1016/j.foodres.2022.111133. View

13.

Kang H . Sample size determination and power analysis using the G*Power software. J Educ Eval Health Prof. 2021; 18:17. PMC: 8441096. DOI: 10.3352/jeehp.2021.18.17. View

14.

Callahan B, McMurdie P, Rosen M, Han A, Johnson A, Holmes S . DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016; 13(7):581-3. PMC: 4927377. DOI: 10.1038/nmeth.3869. View

15.

Jayabalan R, Malbasa R, Loncar E, Vitas J, Sathishkumar M . A Review on Kombucha Tea-Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus. Compr Rev Food Sci Food Saf. 2021; 13(4):538-550. DOI: 10.1111/1541-4337.12073. View

16.

Atkinson F, Cohen M, Lau K, Brand-Miller J . Glycemic index and insulin index after a standard carbohydrate meal consumed with live kombucha: A randomised, placebo-controlled, crossover trial. Front Nutr. 2023; 10:1036717. PMC: 9982099. DOI: 10.3389/fnut.2023.1036717. View

17.

Wang L, Jiang L, Chu Y, Feng F, Tang W, Chen C . Dietary Taurine Improves Growth Performance and Intestine Health via the GSH/GSSG Antioxidant System and Nrf2/ARE Signaling Pathway in Weaned Piglets. Antioxidants (Basel). 2023; 12(10). PMC: 10604690. DOI: 10.3390/antiox12101852. View

18.

Xiang N, Xu L, Qian H, Zhang D . Multiple obesity indices suggest a close relationship between obesity and constipation: evidence from NHANES. BMC Public Health. 2024; 24(1):1273. PMC: 11084052. DOI: 10.1186/s12889-024-18647-y. View

19.

Wang P, Feng Z, Sang X, Chen W, Zhang X, Xiao J . Kombucha ameliorates LPS-induced sepsis in a mouse model. Food Funct. 2021; 12(20):10263-10280. DOI: 10.1039/d1fo01839f. View

20.

Zeng Q, Li D, He Y, Li Y, Yang Z, Zhao X . Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities. Sci Rep. 2019; 9(1):13424. PMC: 6748942. DOI: 10.1038/s41598-019-49462-w. View