Ultra-Selective and Sensitive Fluorescent Chemosensor Based on Phage Display-Derived Peptide with an N-Terminal Cu(II)-Binding Motif

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2024 Nov 26

PMID 39590014

Authors

Marta Sosnowska

Tomasz Lega

Dawid Nidzworski

Marcin Olszewski

Beata Gromadzka

Affiliations

Soon will be listed here.

Abstract

Copper, along with gold, was among the first metals that humans employed. Thus, the copper pollution of the world's water resources is escalating, posing a significant threat to human health and aquatic ecosystems. It is crucial to develop detection technology that is both low-cost and feasible, as well as ultra-selective and sensitive. This study explored the use of the NH-Xxx-His motif-derived peptide from phage display technology for ultra-selective Cu detection. Various Cu-binding M13 phage clones were isolated, and their affinity and cross-reactivity for different metal ions were determined. A detailed analysis of the amino acid sequence of the unique Cu-binding peptides was employed. For the development of an optical chemosensor, a peptide with an NH-Xxx-His motif was selected. The dansyl group was incorporated during solid-phase peptide synthesis, and fluorescence detection assays were employed. The efficacy of the Cu-binding peptide was verified through spectroscopic measurements. In summary, we developed a highly selective and sensitive fluorescent chemosensor for Cu detection based on a peptide sequence from a phage display library that carries the N-terminal Xxx-His motif.

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