Exploring Botryosphaeran, a (1 → 3)(1 → 6)-β-D-Glucan, As a Matrix for the Stabilization of Laccase from Pleurotus Ostreatus Florida Onto a Zinc Oxide Quantum Dots Platform for the Electrochemical Determination of 2,6-Dimethoxyphenol

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2024 Nov 25

PMID 39585553

Authors

Bruna Coldibeli

Gabriel J Mattos

Gustavo Fix

Gabriel R P Manrique

Aneli M Barbosa-Dekker

Robert F H Dekker

Elen R Sartori

Affiliations

Soon will be listed here.

Abstract

This work describes the development of a novel biosensor obtained by immobilizing laccase from Pleurotus ostreatus Florida onto a glassy carbon electrode platform modified with zinc oxide quantum dots. For enzyme immobilization, the exopolysaccharide botryosphaeran from Botryosphaeria rhodina MAMB-05 was used. Although both biomaterials are from different fungal sources, laccase immobilization was guaranteed, which was demonstrated by the excellent stability of the fabricated biosensor device for the voltammetric determination of 2,6-dimethoxyphenol (2,6-DMP). Under the optimal experimental conditions, the cathodic current from the square-wave voltammograms presented a linear dependence on the 2,6-DMP concentration within the range of 10-400 nmol L, with a limit of detection of 9 nmol L. This bioanalytical device exhibited excellent repeatability and long-term storage stability.

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