Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents

Overview

Journal J Aging Res

Publisher Wiley

Specialty Geriatrics

Date 2024 Nov 25

PMID 39583064

Authors

Insun Song

Pil-Jong Kim

Yong Jun Choi

Yoon-Sok Chung

Soonchul Lee

Jeong-Hwa Baek

Kyung Mi Woo

Affiliations

Soon will be listed here.

Abstract

This study identifies senescent osteocytes in the femur and tibia of young rodents and explores their role in bone remodeling. The proximity of osteoclasts to senescent osteocytes was observed, which is a new finding. Cultured osteocytes, sorted using a podoplanin antibody in FACS, exhibited osteocytic characteristics and increased senescence-related genes. Senescent osteocytes secreted cytokines associated with senescence, remodeling, and inflammation. Notably, IGF1 and MMP2 were elevated in podoplanin-positive (pdpn) osteocytes. Migration assays demonstrated significant osteoclast precursor migration towards senescent osteocytes, further confirmed by co-culture experiments leading to osteoclast differentiation. These findings suggest that senescent osteocytes have a pivotal role in initiating bone resorption, with recruitment of osteoclast precursors during early bone remodeling stages. In conclusion, our research enhances our understanding of complicated bone remodeling mechanisms and bone homeostasis.

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